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Buccutite™ FOL scavenger

Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient.
Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient.
Ordering information
Price ()
Catalog Number5360
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight402.50
SolventDMSO
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
402.50
Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. This Buccutite™ FOL reagent is used to eliminate unreacted FOL groups for the crosslinked product if desired. For the vast majority of applications, there is no need to block the unreacted FOL groups from the final crosslinked product.

Example protocol


AT A GLANCE

  1. Prepare Buccutite™ FOL scavenger stock solution.
  2. Add 1 uL /100 uL reaction mixture.
  3. Incubate at room temperature for 30~60 minutes.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Add 200 uL DMSO to Buccutite™ FOL scavenger vial to prepare 10 mM stock solution. Note: The Buccutite™ FOL scavenger stock solution should be stored at -20 °C after preparation and stable for 2 months if avoid repeated freeze-thaw cycles.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Add 1 uL /100 uL reaction mixture so the final concentration of Buccutite™ FOL scavenger in reaction mixture is 100 uM.

  2. Incubate at room temperature for 30~60 minutes. Note: 1 uL Buccutite™ FOL scavengerstock solution is enough to scavenge  FOL groups in solution when the concentration is lower than 100 uM. If there is higher FOL groups in the mixture, the volume needs to be adjusted to scavenge all the FOL groups.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Buccutite™ FOL scavenger to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM248.447 µL1.242 mL2.484 mL12.422 mL24.845 mL
5 mM49.689 µL248.447 µL496.894 µL2.484 mL4.969 mL
10 mM24.845 µL124.224 µL248.447 µL1.242 mL2.484 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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