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Buccutite™ MTA, maleimide [MTAM]

Chemical structure for Buccutite™ MTA, maleimide [MTAM].
Chemical structure for Buccutite™ MTA, maleimide [MTAM].
Ordering information
Price ()
Catalog Number5358
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. The method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL under neutral conditions. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. Buccutite™ MTA maleimide (MTAM) can be used the same way as the widely used SMCC for crosslinking proteins. One end of the MTAM reacts (via maleimide) with thiols (-SH) of cysteine found in the reduced antibodies (by TCEP or DTT). SMCC crosslinking requires high concentration of proteins. In addition, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. Buccutite™ crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient.

References


View all 50 references: Citation Explorer
DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria.
Authors: Chesner, Lisa N and Essawy, Maram and Warner, Cecilia and Campbell, Colin
Journal: DNA repair (2021): 103026
Previously unknown type of protein crosslink discovered.
Authors: Fass, Deborah and Semenov, Sergey N
Journal: Nature (2021): 343-344
DNA-protein crosslink proteases in genome stability.
Authors: Ruggiano, Annamaria and Ramadan, Kristijan
Journal: Communications biology (2021): 11
The Trinity of SPRTN Protease Regulation.
Authors: Ruggiano, Annamaria and Ramadan, Kristijan
Journal: Trends in biochemical sciences (2021): 2-4
Protein-oligonucleotide conjugates as model substrates for DNA-protein crosslink repair proteases.
Authors: Reinking, Hannah K and Stingele, Julian
Journal: STAR protocols (2021): 100591
The Ubiquitin Ligase TRAIP: Double-Edged Sword at the Replisome.
Authors: Wu, R Alex and Pellman, David S and Walter, Johannes C
Journal: Trends in cell biology (2021): 75-85
A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN.
Authors: Zhao, Shubo and Kieser, Anja and Li, Hao-Yi and Reinking, Hannah K and Weickert, Pedro and Euteneuer, Simon and Yaneva, Denitsa and Acampora, Aleida C and Götz, Maximilian J and Feederle, Regina and Stingele, Julian
Journal: Nucleic acids research (2021): 902-915
Emerging roles of Wss1 in the survival of Candida albicans under genotoxic stresses.
Authors: Homchan, Aimorn and Sukted, Juthamas and Matangkasombut, Oranart and Pakotiprapha, Danaya
Journal: Current genetics (2021): 99-105
Treatment of human cells with 5-aza-dC induces formation of PARP1-DNA covalent adducts at genomic regions targeted by DNMT1.
Authors: Kiianitsa, Kostantin and Zhang, Yinbo and Maizels, Nancy
Journal: DNA repair (2020): 102977
A Structure-Based Mechanism for DNA Entry into the Cohesin Ring.
Authors: Higashi, Torahiko L and Eickhoff, Patrik and Sousa, Joana S and Locke, Julia and Nans, Andrea and Flynn, Helen R and Snijders, Ambrosius P and Papageorgiou, George and O'Reilly, Nicola and Chen, Zhuo A and O'Reilly, Francis J and Rappsilber, Juri and Costa, Alessandro and Uhlmann, Frank
Journal: Molecular cell (2020): 917-933.e9