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Buccutite™ MTA, maleimide [MTAM]

Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. The method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL under neutral conditions. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. Buccutite™ MTA maleimide (MTAM) can be used the same way as the widely used SMCC for crosslinking proteins. One end of the MTAM reacts (via maleimide) with thiols (-SH) of cysteine found in the reduced antibodies (by TCEP or DTT). SMCC crosslinking requires high concentration of proteins. In addition, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. Buccutite™ crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient.

References

View all 50 references: Citation Explorer
DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria.
Authors: Chesner, Lisa N and Essawy, Maram and Warner, Cecilia and Campbell, Colin
Journal: DNA repair (2021): 103026
Emerging roles of Wss1 in the survival of Candida albicans under genotoxic stresses.
Authors: Homchan, Aimorn and Sukted, Juthamas and Matangkasombut, Oranart and Pakotiprapha, Danaya
Journal: Current genetics (2021): 99-105
A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN.
Authors: Zhao, Shubo and Kieser, Anja and Li, Hao-Yi and Reinking, Hannah K and Weickert, Pedro and Euteneuer, Simon and Yaneva, Denitsa and Acampora, Aleida C and Götz, Maximilian J and Feederle, Regina and Stingele, Julian
Journal: Nucleic acids research (2021): 902-915
The Ubiquitin Ligase TRAIP: Double-Edged Sword at the Replisome.
Authors: Wu, R Alex and Pellman, David S and Walter, Johannes C
Journal: Trends in cell biology (2021): 75-85
Protein-oligonucleotide conjugates as model substrates for DNA-protein crosslink repair proteases.
Authors: Reinking, Hannah K and Stingele, Julian
Journal: STAR protocols (2021): 100591
Page updated on November 12, 2024

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Catalog Number5358
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Physical properties

Molecular weight

1151

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Flow cytometry analysis of whole blood stained with PE-iFluor®610 anti-human CD8 *SK1* conjugates. Two different methods were used prepared the conjugates: the SMCC method and the Buccutite™ MTA-Maleimide method. The fluorescence signal was monitored using an Aurora spectral flow cytometer in B6-A channel. Top) Flow cytometry data was generated using a 4-laser (355 nm, 405 nm, 488 nm, and 640 nm) spectral cytometer. Bottom) CD8+ signal intensity in B6-A channel, Stain Index and Yield was compared between two methods.
Flow cytometry analysis of whole blood stained with PE-iFluor®610 anti-human CD8 *SK1* conjugates. Two different methods were used prepared the conjugates: the SMCC method and the Buccutite™ MTA-Maleimide method. The fluorescence signal was monitored using an Aurora spectral flow cytometer in B6-A channel. Top) Flow cytometry data was generated using a 4-laser (355 nm, 405 nm, 488 nm, and 640 nm) spectral cytometer. Bottom) CD8+ signal intensity in B6-A channel, Stain Index and Yield was compared between two methods.
Flow cytometry analysis of whole blood stained with PE-iFluor®610 anti-human CD8 *SK1* conjugates. Two different methods were used prepared the conjugates: the SMCC method and the Buccutite™ MTA-Maleimide method. The fluorescence signal was monitored using an Aurora spectral flow cytometer in B6-A channel. Top) Flow cytometry data was generated using a 4-laser (355 nm, 405 nm, 488 nm, and 640 nm) spectral cytometer. Bottom) CD8+ signal intensity in B6-A channel, Stain Index and Yield was compared between two methods.
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