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Buccutite™ MTA, maleimide
MTAM
Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. The method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL under neutral conditions. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. Buccutite™ MTA maleimide (MTAM) can be used the same way as the widely used SMCC for crosslinking proteins. One end of the MTAM reacts (via maleimide) with thiols (-SH) of cysteine found in the reduced antibodies (by TCEP or DTT). SMCC crosslinking requires high concentration of proteins. In addition, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. Buccutite™ crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient.
Flow cytometry analysis of whole blood stained with PE-iFluor®610 anti-human CD8 *SK1* conjugates. Two different methods were used prepared the conjugates: the SMCC method and the Buccutite™ MTA-Maleimide method. The fluorescence signal was monitored using an Aurora spectral flow cytometer in B6-A channel. Top) Flow cytometry data was generated using a 4-laser (355 nm, 405 nm, 488 nm, and 640 nm) spectral cytometer. Bottom) CD8+ signal intensity in B6-A channel, Stain Index and Yield was compared between two methods.
Flow cytometry analysis of whole blood stained with PE-iFluor®610 anti-human CD8 *SK1* conjugates. Two different methods were used prepared the conjugates: the SMCC method and the Buccutite™ MTA-Maleimide method. The fluorescence signal was monitored using an Aurora spectral flow cytometer in B6-A channel. Top) Flow cytometry data was generated using a 4-laser (355 nm, 405 nm, 488 nm, and 640 nm) spectral cytometer. Bottom) CD8+ signal intensity in B6-A channel, Stain Index and Yield was compared between two methods.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
53582 umoles
Price
 
Physical properties
Molecular weight1151
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 7, 2025