Buccutite™ MTA scavenger

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	690.96
Solvent	DMSO

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

690.96

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient. Many of our customer have requested us to offer the stand-alone Buccutite™ MTA and Buccutite™ FOL reagents to expand the application of Buccutite™crosslinking technology. This Buccutite™ MTA reagent is used to eliminate unreacted MTA groups of the crosslinked product if desired. For the vast majority of applications, there is no need to block the unreacted MTA groups from the final crosslinked product.

Example protocol

AT A GLANCE

Prepare Buccutite™ MTA scavenger stock solution.
Add 1 uL /100 uL reaction mixture.
Incubate at room temperature for 30~60 minutes.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Add 200uL DMSO to Buccutite™ MTA scavenger vial to prepare 10 mM stock solution. Note: The Buccutite™ MTA scavenger stock solution should be stored at -20 °C after preparation and stable for 2 months if avoid repeated freeze-thaw cycles.

SAMPLE EXPERIMENTAL PROTOCOL

Add 1 uL /100 uL reaction mixture so the final concentration of Buccutite™ MTA scavenger in reaction mixture is 100 uM.
Incubate at room temperature for 30~60 minutes. Note: 1 uL Buccutite™ MTA scavenger stock solution is enough to scavenge MTA groups in solution when the concentration is lower than 100 uM. If there is higher concentration -MTA in the mixture, the volume needs to be adjusted to scavenge all the MTA groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Buccutite™ MTA scavenger to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	144.726 µL	723.631 µL	1.447 mL	7.236 mL	14.473 mL
5 mM	28.945 µL	144.726 µL	289.452 µL	1.447 mL	2.895 mL
10 mM	14.473 µL	72.363 µL	144.726 µL	723.631 µL	1.447 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one molecule, while FOL is added to another molecule. The cross-linking reaction is initiated by mixing Molecule-1-Buccutite ™ MTA and Molecule-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild and neutral conditions without any catalyst required. It is robust and efficient.