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Buccutite™ PolyHRP Antibody Conjugation Kit
Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level. Buccutite™ PolyHRP Antibody Conjugation Kit is designed for rapid preparation of poly-HRP conjugated antibodies. The kit provides the poly-HRP that is pre-activated with our proprietary linker Buccutite™ FOL. A targeted antibody is activated with Buccutite™ MTA (provided in the kit) to give MTA-activated antibody. The simple mix of MTA-activated antibody with FOL-activated poly-HRP to give the desired poly-HRP-labeled antibody conjugate. The reaction occurs under extremely mild conditions without a catalyst required. The poly-HRP antibody conjugate prepared with Buccutite™ PolyHRP Antibody Conjugation Kit is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, western blotting, immunohistochemistry (IHC) assays. It can also be used with TSA or our Styramide™ fluorescent HRP substrates for ultrasensitive detection of low abundant biological targets.
Direct ELISA curves were generated using the polyHRP- goat-anti-mouse IgG conjugate prepared with Buccutite&trade; Poly-HRP Antibody Conjugation Kit (Cat No. 5518, Cat No. 5519). A 3-fold&nbsp; serial diluted mouse IgG was coated onto a 96-well plate, and 100 &micro;L GAM IgG-polyHRP conjugate (100 ng/ml) was tested using the standard ELISA method. TMB substrate solution (Cat No. 11003) was used to detect the immobilized mouse IgG with 5 min incubation and read at 450 nm.<br><br />Blue: Buccutite&trade; Kit 5518 or 5519<br />Red: GXM IgG PolyHRP (Vendor A)<br />Green: GXM IgG-HRP (Vendor B)
Direct ELISA curves were generated using the polyHRP- goat-anti-mouse IgG conjugate prepared with Buccutite&trade; Poly-HRP Antibody Conjugation Kit (Cat No. 5518, Cat No. 5519). A 3-fold&nbsp; serial diluted mouse IgG was coated onto a 96-well plate, and 100 &micro;L GAM IgG-polyHRP conjugate (100 ng/ml) was tested using the standard ELISA method. TMB substrate solution (Cat No. 11003) was used to detect the immobilized mouse IgG with 5 min incubation and read at 450 nm.<br><br />Blue: Buccutite&trade; Kit 5518 or 5519<br />Red: GXM IgG PolyHRP (Vendor A)<br />Green: GXM IgG-HRP (Vendor B)
protocol
Protocol
CatalogSize
Price
Quantity
55181x50 ug Labeling
Price
55192x50 ug Labelings
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Documents
Protocol
Safety Data Sheet (Catalog 5518)
Safety Data Sheet (Catalog 5519)
Certificate of Analysis
Brochure: Buccutite™ Crosslinking Technology
Brochure: Labeling Antibodies & Biopolymers
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Page updated on December 15, 2025