logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Buccutite™ Rapid APC Antibody Labeling Kit
Microscale Optimized for Labeling 100 ug Antibody Per Reaction
APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ APC Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. The kit includes a preactivated APC and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC to facilitate the APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Flow cytometry analysis of CD4 PBMC populations. Anti-human CD4 monoclonal antibody was labeled using Buccutite™ Rapid APC Antibody Labeling Kit (Cat No. 1311) or Lightning-Link® Rapid APC Antibody Labeling Kit according to manufacturers’ instructions. CD4 PBMC populations were then stained and the fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC channel.
Flow cytometry analysis of CD4 PBMC populations. Anti-human CD4 monoclonal antibody was labeled using Buccutite™ Rapid APC Antibody Labeling Kit (Cat No. 1311) or Lightning-Link® Rapid APC Antibody Labeling Kit according to manufacturers’ instructions. CD4 PBMC populations were then stained and the fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC channel.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
13112 Labelings
Price
 
Spectral properties
Correction factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Buccutite™ Crosslinking Technology
Brochure: Labeling Antibodies & Biopolymers
Brochure: Phycobiliproteins & Tandem Conjugates
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 29, 2025