Name: Buccutite™ Rapid APC Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*
Brand: AAT Bioquest
SKU: 1311
Price: 582 USD
Availability: InStock

Buccutite™ Rapid APC Antibody Labeling Kit

Microscale Optimized for Labeling 100 ug Antibody Per Reaction

APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ APC Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. The kit includes a preactivated APC and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC to facilitate the APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

Flow cytometry analysis of CD4 PBMC populations. Anti-human CD4 monoclonal antibody was labeled using Buccutite™ Rapid APC Antibody Labeling Kit (Cat No. 1311) or Lightning-Link® Rapid APC Antibody Labeling Kit according to manufacturers’ instructions. CD4 PBMC populations were then stained and the fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1311	2 Labelings	Price

References

View all 46 references: Citation Explorer

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120

Authors:

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.

Journal:

J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

Authors:

Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

Journal:

Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin

Authors:

Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.

Journal:

Biophys J (2004): 2598

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp

Authors:

Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.

Journal:

Acta Biol Hung (2003): 113

Evaluation of Tolypothrix germplasm for phycobiliprotein content

Authors:

Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.

Journal:

Folia Microbiol (Praha) (2003): 59

Spectral properties

Correction factor (280 nm)	0.195
Extinction coefficient (cm ^-1 M ^-1)	730000
Excitation (nm)	651
Emission (nm)	660

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

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Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025

Component A: Buccutite™ FOL-Activated APC	2 vials (lyophilized)
Component B: Buccutite™ MTA	2 vials (lyophilized)
Component C: Reaction Buffer	1 Vial (20 µL)