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Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labeling Kit
Microscale Optimized for Labeling 100 ug Antibody Per Reaction
APC-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~700 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ APC-Cy5.5 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. The kit includes a preactivated APC-Cy5.5 tandem and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC-Cy5.5 tandem to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
<p>AAT Bioquest offers this Buccutite&trade; rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.&nbsp; Our preactivated APC was premodified with our Buccutite&trade; FOL. Your antibody (or other proteins) is modified with our Buccutite&trade; MTA to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC to give the desired APC-antibody conjugate in much higher yield than the SMCC chemistry. In addition, our preactivated APC reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>
<p>AAT Bioquest offers this Buccutite&trade; rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.&nbsp; Our preactivated APC was premodified with our Buccutite&trade; FOL. Your antibody (or other proteins) is modified with our Buccutite&trade; MTA to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC to give the desired APC-antibody conjugate in much higher yield than the SMCC chemistry. In addition, our preactivated APC reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
13202 Lalelings
Price
 
Spectral properties
Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)651
Emission (nm)700
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Buccutite™ Crosslinking Technology
Brochure: Labeling Antibodies & Biopolymers
Brochure: Phycobiliproteins & Tandem Conjugates
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Page updated on October 30, 2025