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Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labeling Kit
Microscale Optimized for Labeling 25 ug Antibody Per Reaction
APC-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~700 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ APC-Cy5.5 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC-Cy5.5 tandem. The kit includes a preactivated APC-Cy5.5 tandem and reaction buffer. The entire process only requires two simple mixings without further purification required. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 25 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC-Cy5.5 tandem to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
<p>AAT Bioquest offers this Buccutite&trade; rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.&nbsp; Our preactivated APC was premodified with our Buccutite&trade; FOL. Your antibody (or other proteins) is modified with our Buccutite&trade; MTA to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC to give the desired APC-antibody conjugate in much higher yield than the SMCC chemistry. In addition, our preactivated APC reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>
<p>AAT Bioquest offers this Buccutite&trade; rapid labeling kit to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.&nbsp; Our preactivated APC was premodified with our Buccutite&trade; FOL. Your antibody (or other proteins) is modified with our Buccutite&trade; MTA to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC to give the desired APC-antibody conjugate in much higher yield than the SMCC chemistry. In addition, our preactivated APC reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13502 Labelings
Price
 
Spectral properties
Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)651
Emission (nm)700
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 13, 2025