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Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kit
Microscale Optimized for Labeling 100 ug Antibody Per Reaction
APC-iFluor® 700 Tandem is an excellent replacement for APC-Alexa Fluor® 700 Tandem since they have almost identical spectra. On some antibodies, our APC-iFluor® 700 Tandem is much brighter than APC-Alexa Fluor® 700 Tandem with a higher stain index. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC-iFluor® 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ APC-iFluor® 700 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. The kit includes a preactivated APC-iFluor® 700 tandem and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC-iFluor® 700 tandem to facilitate the APC-iFluor® 700 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-iFluor® 700 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-iFluor® 700 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Flow cytometry analysis of whole blood stained with APC-iFluor® 700 anti-human CD4 *SK3* conjugate prepared using the Buccutite™ Rapid iFluor® 700 Tandem Antibody Labeling Kit (1319). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700-specific R4-A channel.
Flow cytometry analysis of whole blood stained with APC-iFluor® 700 anti-human CD4 *SK3* conjugate prepared using the Buccutite™ Rapid iFluor® 700 Tandem Antibody Labeling Kit (1319). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-iFluor® 700-specific R4-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13192 Labelings
Price
 
Spectral properties
Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)651
Emission (nm)710
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 13, 2025