Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kit *Production Scale Optimized for Labeling 1 mg Antibody Per Reaction*

Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kit

Production Scale Optimized for Labeling 1 mg Antibody Per Reaction

Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kit enables efficient, production-scale fluorescent labeling for flow cytometry and immunoassay applications.

APC-iFluor® 700 Labeling: Preactivated tandem dye with 651 nm excitation and 710 nm emission.
Conjugation Chemistry: Two-step FOL/MTA labeling completes conjugation in under 2 hours.
High Conjugation Efficiency: Robust conjugation with spin-column purification for high conjugate yield.
Production-Scale Labeling Format: Supports two labeling reactions of up to 1 mg antibody each.
Applications: Demonstrated for CD4 detection using Cytek Aurora spectral flow cytometry.

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kit (Cat. #5413). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the R4-A channel, demonstrating clear detection of CD4⁺ cells.

Protocol

SDS

Catalog	Size	Price	Quantity
5413	2 Labelings	Price

Overview

Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kits, designed for large-scale production, provide a streamlined approach for labeling antibodies with PE, APC, PerCP, and iFluor® tandem dyes. Compared to conventional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate APC-iFluor® 700 to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column for maximum conjugate yield. Each Buccutite™ FOL-Activated APC-iFluor® 700 vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Allophycocyanin-iFluor® 700 (APC-iFluor® 700) is an intensely bright, NIR fluorescent tandem fluorophore with an excitation and emission maxima of ~651 nm and ~710 nm, respectively. Given its intense brightness, APC-iFluor® 700 is recommended for pairing with low-abundance targets to minimize spillover and compensation. APC-iFluor® 700 conjugates are well-suited for flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.

References

View all 19 references: Citation Explorer

Circulating Regulatory T Cell Subsets in Patients with Sarcoidosis.

Authors:

Kudryavtsev, Igor and Zinchenko, Yulia and Starshinova, Anna and Serebriakova, Maria and Malkova, Anna and Akisheva, Tatiana and Kudlay, Dmitriy and Glushkova, Anzhela and Yablonskiy, Piotr and Shoenfeld, Yehuda

Journal:

Diagnostics (Basel, Switzerland) (2023)

Development of Porcine Monoclonal Antibodies with In Vitro Neutralizing Activity against Classical Swine Fever Virus from C-Strain E2-Specific Single B Cells.

Authors:

Wang, Lihua and Madera, Rachel and Li, Yuzhen and Gladue, Douglas P and Borca, Manuel V and McIntosh, Michael T and Shi, Jishu

Journal:

Viruses (2023)

Deciphering the role of the conjugate's phycoerythrin label in complement-mediated interference occurring in HLA single antigen Luminex bead assays.

Authors:

Devriese, Magali and Hays, Constantin and Jouffrey, Julie and Usureau, Cédric and Carmagnat, Maryvonnick and Caillat-Zucman, Sophie and Taupin, Jean Luc

Journal:

HLA (2022): 573-579

Natural exosome-like nanovesicles from edible tea flowers suppress metastatic breast cancer via ROS generation and microbiota modulation.

Authors:

Chen, Qiubing and Li, Qian and Liang, Yuqi and Zu, Menghang and Chen, Nanxi and Canup, Brandon S B and Luo, Liyong and Wang, Chenhui and Zeng, Liang and Xiao, Bo

Journal:

Acta pharmaceutica Sinica. B (2022): 907-923

iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility.

Authors:

Jensen, Holly A and Kim, Jeong

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022)

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	710

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 25, 2026

Component A: Buccutite™ FOL-Activated APC-iFluor® 700	2 vials
Component B: Buccutite™ MTA	2 vials
Component C: Reaction Buffer	1 vial (100 µL)
Component D: Spin Column	2 columns