Buccutite™ Rapid APC-iFluor® 750 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*

Buccutite™ Rapid APC-iFluor® 750 Tandem Antibody Labeling Kit

Microscale Optimized for Labeling 25 ug Antibody Per Reaction

Buccutite™ Rapid APC-iFluor® 750 Tandem Antibody Labeling Kit facilitates the APC-iFluor® 750 tandem conjugations to antibodies and other proteins for flow cytometry and immunoassay applications.

APC-iFluor® 750 Labeling: Preactivated tandem dye with 651 nm excitation and 783 nm emission.
Conjugation Chemistry: Uses FOL/MTA chemistry for amino group-based antibody labeling.
High Conjugation Efficiency: Higher yields than conventional SMCC-based chemistry.
Microscale Labeling Format: Supports two labeling reactions of up to 25 µg antibody each.
Applications: Suitable for flow cytometry, Western blotting, ELISA, and immunohistochemistry.

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid APC-iFluor® 750 Tandem Antibody Labeling Kit (Cat. #1348). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the R7-A channel, demonstrating clear detection of CD4⁺ cells.

Protocol

SDS

Catalog	Size	Price	Quantity
1348	2 Labelings	Price

Overview

APC-iFluor® 750 Tandem is an excellent replacement for APC-Cy7 and APC-Alexa Fluor® 750 Tandem since they have almost identical spectra. On some antibodies, our APC-iFluor® 750 Tandem is much brighter than APC-Cy7, APC-H7, and APC-Alexa Fluor® 750 Tandem with a higher stain index. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC-iFluor® 750 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ APC-iFluor® 750 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC-iFluor® 750 tandem. The kit includes a preactivated APC-iFluor® 750 tandem and reaction buffer. The entire process only requires two simple mixings without further purification required. The conjugated antibody can be used in WB, ELISA, and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 25 µg of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC-iFluor® 750 tandem to facilitate the APC-iFluor® 750 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-iFluor® 750 tandem is ready to conjugate, giving a much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-iFluor® 750 tandem is conjugated to a protein via its amino group that is abundant in proteins, while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

References

View all 3 references: Citation Explorer

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.

Authors:

Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90

Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets.

Authors:

Mittag, Anja and Lenz, Dominik and Gerstner, Andreas O H and Sack, Ulrich and Steinbrecher, Michael and Koksch, Mario and Raffael, Alexander and Bocsi, Jozsef and Tárnok, Attila

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 103-15

Near-infrared dyes for six-color immunophenotyping by laser scanning cytometry.

Authors:

Gerstner, Andreas O H and Lenz, Dominik and Laffers, Wiebke and Hoffman, Robert A and Steinbrecher, Michael and Bootz, Friedrich and Tárnok, Attila

Journal:

Cytometry (2002): 115-23

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	783

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 25, 2026

Component A: Buccutite™ FOL-Activated APC-iFluor® 750	2 vials
Component B: Buccutite™ MTA	1 vial
Component C: Reaction Buffer	1 vial (20 µL)