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Buccutite™ Rapid Crosslinked APC Antibody Labeling Kit
Production Scale Optimized for Labeling 1 mg Antibody Per Reaction
Buccutite™ Rapid Crosslinked APC Antibody Labeling Kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with APC, PE, and iFluor® tandem dyes. In comparison to traditional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate APC to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column to maximize conjugate yield. Each Buccutite™ FOL-Activated APC vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Allophycocyanin (APC) is an intensely bright, red fluorescent phycobiliprotein with an excitation and emission maxima of ~651 nm and ~660 nm, respectively. Given its intense brightness, APC is recommended for pairing with low-abundance targets to minimize spillover and compensation. APC conjugates are well-suited for FRET screening, flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
Flow cytometry analysis of CD4 PBMC populations. Anti-human CD4 monoclonal antibody was labeled using Buccutite™ Rapid Crosslinked APC Antibody Labeling Kit *Production Scale* (Cat No. 5406) or Lightning-Link® Rapid APC Antibody Labeling Kit according to manufacturers’ instructions. CD4 PBMC populations were then stained, and the fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC channel.
Flow cytometry analysis of CD4 PBMC populations. Anti-human CD4 monoclonal antibody was labeled using Buccutite™ Rapid Crosslinked APC Antibody Labeling Kit *Production Scale* (Cat No. 5406) or Lightning-Link® Rapid APC Antibody Labeling Kit according to manufacturers’ instructions. CD4 PBMC populations were then stained, and the fluorescence signal was monitored using an ACEA NovoCyte flow cytometer in the APC channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
54062 Labelings
Price
 
Spectral properties
Correction factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 20, 2025