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Buccutite™ Rapid Crosslinked APC Antibody Labeling Kit
Production Scale Optimized for Labeling 1 mg Antibody Per Reaction
Buccutite™ Rapid Crosslinked APC Antibody Labeling Kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with APC, PE, and iFluor® tandem dyes. In comparison to traditional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate APC to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column to maximize conjugate yield. Each Buccutite™ FOL-Activated APC vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Allophycocyanin (APC) is an intensely bright, red fluorescent phycobiliprotein with an excitation and emission maxima of ~651 nm and ~660 nm, respectively. Given its intense brightness, APC is recommended for pairing with low-abundance targets to minimize spillover and compensation. APC conjugates are well-suited for FRET screening, flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid APC Antibody Labeling Kit (Cat. #5406). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the R1-A channel, demonstrating clear detection of CD4⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid APC Antibody Labeling Kit (Cat. #5406). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the R1-A channel, demonstrating clear detection of CD4⁺ cells.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
54062 Labelings
Price
 
Spectral properties
Correction factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on June 18, 2026