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Buccutite™ Rapid Oligo Antibody Conjugation Kit
Optimized for Labeling 100 ug Protein

Buccutite™ Rapid Oligo Antibody Conjugation Kit is optimized for conjugating 100 ug protein with an amino-modified oligo. The oligonucleotide conjugation kit enables the easy and efficient generation of antibody-oligonucleotide conjugates. The Buccutite crosslinking technique has been proven to be one of the most effective conjugation methods for crosslinking two large molecules. The kit is one of the most effective oligo-antibody conjugation products. It can be used to generate conjugates of different ratios of antibody/oligonucleotide. It can be used to conjugate antibodies to single-stranded or double-stranded oligonucleotides that are modified with an amine group, typically on a terminal. It is quick and easy to use with high levels of antibody and oligo recovery. It is flexible to control the ratio of oligo/antibody at your desired level. The resulting conjugate is highly stable since the oligo and antibody are covalently connected via the highly stable amide bond. It can be used for a wide range of target proteins, including antibody fragments and small proteins. Antibody-oligonucleotide conjugates (AOCs) belong to a class of chimeric molecules combining two important families of biomolecules: antibodies and oligonucleotides. Combination of exceptional targeting capabilities of antibodies with numerous functional modalities of oligonucleotides has been fruitful for a variety of applications with AOC including single cell sequencing, imaging, detection and targeted therapeutics.

The Buccutite™ Rapid Oligo Antibody Conjugation kit (AAT Cat# 5450) was utilized to conjugate rabbit IgG with a 20-nucleotide-containing oligo. The conjugation was confirmed using a 4-12% reducing gel SDS-PAGE. The reaction mix ratios of antibody:oligo examined were 5X, 10X, and 20X, as well as the reactions of oligo + protein (O+P), protein alone (P), and oligo alone (O).
The Buccutite™ Rapid Oligo Antibody Conjugation kit (AAT Cat# 5450) was utilized to conjugate rabbit IgG with a 20-nucleotide-containing oligo. The conjugation was confirmed using a 4-12% reducing gel SDS-PAGE. The reaction mix ratios of antibody:oligo examined were 5X, 10X, and 20X, as well as the reactions of oligo + protein (O+P), protein alone (P), and oligo alone (O).
CatalogSize
Price
Quantity
54502 Labelings
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on November 20, 2025