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Buccutite™ Rapid PE Antibody Labeling Kit
Microscale Optimized for Labeling 100 ug Antibody Per Reaction
R-Phycoerythrin (PE) is an orange fluorescent protein which has an excitation wavelength of 565 nm and an emission wavelength of 575 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the PE conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ PE Conjugation Kit provides a robust and convenient method to conjugate your antibodies with PE. The kit includes a preactivated PE and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. Considering the large size of PE (240 kDa), the amount of antibody used in a labeling reaction must always be less than the amount of PE. The best ratio for any new antibody reagent must be determined by experimentation but 50-60 ug of IgG antibody for every 100 ug of PE usually gives optimal results. Our kit provides preactivated PE to facilitate the PE conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid PE Antibody Labeling Kit (Cat. #1310). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B4-A channel, demonstrating clear detection of CD4⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid PE Antibody Labeling Kit (Cat. #1310). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B4-A channel, demonstrating clear detection of CD4⁺ cells.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
13102 Labelings
Price
 
Spectral properties
Correction factor (280 nm)0.175
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)574
Quantum yield
0.82
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on May 14, 2026