logo
Products
Services
Resources
Selection Guides
About
Buccutite™ Rapid PE Antibody Labeling Kit
Production Scale Optimized for Labeling 1 mg Antibody Per Reaction
Buccutite™ Rapid PE Antibody Labeling Kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC, and iFluor® tandem dyes. In comparison to traditional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is more robust and straightforward. Using a two-step mixing protocol, researchers can directly conjugate PE to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column for maximum conjugate yield. Each Buccutite™ FOL-Activated PE vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Phycoerythrin (PE) is an intensely bright, red-orange fluorescent phycobiliprotein with an excitation and emission maxima of ~565 nm and ~576 nm, respectively. Given its intense brightness, PE is recommended for pairing with low-abundance targets to minimize spillover and compensation. PE conjugates are well-suited for flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid PE Antibody Labeling Kit (Cat. #5405). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B4-A channel, demonstrating clear detection of CD4⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with Anti-human CD4 Antibody (SK3) labeled using Buccutite™ Rapid PE Antibody Labeling Kit (Cat. #5405). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B4-A channel, demonstrating clear detection of CD4⁺ cells.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
54052 Labelings
Price
 
Physical properties
SolventDMSO
Spectral properties
Correction factor (280 nm)0.175
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)574
Quantum yield
0.82
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on June 1, 2026