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Buccutite™ Rapid PE Antibody Labeling Kit
Production Scale Optimized for Labeling 1 mg Antibody Per Reaction
Buccutite™ Rapid PE Antibody Labeling Kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC, and iFluor® tandem dyes. In comparison to traditional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is more robust and straightforward. Using a two-step mixing protocol, researchers can directly conjugate PE to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column for maximum conjugate yield. Each Buccutite™ FOL-Activated PE vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Phycoerythrin (PE) is an intensely bright, red-orange fluorescent phycobiliprotein with an excitation and emission maxima of ~565 nm and ~576 nm, respectively. Given its intense brightness, PE is recommended for pairing with low-abundance targets to minimize spillover and compensation. PE conjugates are well-suited for flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-specific B6-A channel. The PE conjugates were prepared using Buccutite™ Rapid PE Antibody Labeling Kit *Production Scale* (Cat# 5405).
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-specific B6-A channel. The PE conjugates were prepared using Buccutite™ Rapid PE Antibody Labeling Kit *Production Scale* (Cat# 5405).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
54052 Labelings
Price
 
Physical properties
SolventDMSO
Spectral properties
Correction factor (280 nm)0.175
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)574
Quantum yield
0.82
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 20, 2025