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Buccutite™ Rapid PE-Cy5.5 Tandem Antibody Labeling Kit
Microscale Optimized for Labeling 100 ug Antibody Per Reaction
PE-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at~700 nm. The filter sets of 682/33 nm and 695/40 nm are recommended for this tandem color. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the PE-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ PE-Cy5.5 Conjugation Kit provides a robust and convenient method to conjugate your antibodies with PE. The kit includes an activated PE and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. Considering the large size of PE (240 kDa), the amount of antibody used in a labeling reaction must always be less than the amount of RPE. The best ratio for any new antibody reagent must be determined by experimentation but 50-60 ug of IgG antibody for every 100 ug of RPE usually gives optimal results. Our kit provides preactivated PE-Cy5.5 to facilitate the PE-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
<p>AAT Bioquest offers this Buccutite&trade; rapid labeling kit to facilitate the PE conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. &nbsp;Our preactivated PE was premodified with our Buccutite&trade; FOL. Your antibody (or other proteins) is modified with our Buccutite&trade; MTA to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified PE to give the desired PE-antibody conjugate in much higher yield than the SMCC chemistry. In addition, our preactivated PE reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>
<p>AAT Bioquest offers this Buccutite&trade; rapid labeling kit to facilitate the PE conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. &nbsp;Our preactivated PE was premodified with our Buccutite&trade; FOL. Your antibody (or other proteins) is modified with our Buccutite&trade; MTA to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified PE to give the desired PE-antibody conjugate in much higher yield than the SMCC chemistry. In addition, our preactivated PE reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13162 Labelings
Price
 
Spectral properties
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)671
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 8, 2025