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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labeling Kit
Production Scale Optimized for Labeling 1 mg Antibody Per Reaction
Buccutite™ Rapid PE-Texas Red Tandem Antibody Labeling Kits, designed for large-scale production, provide a streamlined approach for labeling antibodies with PE, APC, PerCP, and iFluor® tandem dyes. Compared to conventional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate PE-Texas Red to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column for maximum conjugate yield. Each Buccutite™ FOL-Activated PE-Texas Red vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. Phycoerythrin-Texas Red (PE-Texas Red) is an intensely bright, red fluorescent tandem fluorophore with an excitation and emission maxima of ~565 nm and ~613 nm, respectively. Given its intense brightness, PE-Texas Red is recommended for pairing with low-abundance targets to minimize spillover and compensation. PE-Texas Red conjugates are well-suited for flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
Flow cytometry analysis of whole blood stained with PE-Texas Red anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-Texas Red specific B6-A channel. PE-Texas Red anti-human CD4 *SK3* conjugates were prepared using the Buccutite™ Rapid PE-Texas Red Tandem Antibody Labeling Kit *Production Scale* (Cat# 5412).
Flow cytometry analysis of whole blood stained with PE-Texas Red anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-Texas Red specific B6-A channel. PE-Texas Red anti-human CD4 *SK3* conjugates were prepared using the Buccutite™ Rapid PE-Texas Red Tandem Antibody Labeling Kit *Production Scale* (Cat# 5412).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
54122 Labelings
Price
 
Spectral properties
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)613
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 5, 2025