Buccutite™ Rapid PerCP Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*

Buccutite™ Rapid PerCP Antibody Labeling Kit

Microscale Optimized for Labeling 100 ug Antibody Per Reaction

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the PerCP conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ PerCP Conjugation Kit provides a robust and convenient method to conjugate antibodies with PerCP. The kit includes a preactivated PerCP and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated PerCP to facilitate the PerCP conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PerCP is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PerCP is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

<p>AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the PerCP conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.  Our preactivated PerCP was premodified with our Buccutite™ FOL. Your antibody (or other proteins) is modified with our Buccutite™ MTA to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified PerCP to give the desired PerCP -antibody conjugate in much higher yield than the SMCC chemistry. In addition, our preactivated PerCP reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
1325	2 Labelings	Price

References

View all 46 references: Citation Explorer

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120

Authors:

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.

Journal:

J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

Authors:

Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

Journal:

Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin

Authors:

Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.

Journal:

Biophys J (2004): 2598

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp

Authors:

Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.

Journal:

Acta Biol Hung (2003): 113

Evaluation of Tolypothrix germplasm for phycobiliprotein content

Authors:

Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.

Journal:

Folia Microbiol (Praha) (2003): 59

Spectral properties

Correction factor (280 nm)	0.22
Extinction coefficient (cm ^-1 M ^-1)	406000
Excitation (nm)	477
Emission (nm)	678

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

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Page updated on October 13, 2025

Component A: Buccutite™ FOL-Activated PerCP	2 vials (lyophilized)
Component B: Buccutite™ MTA	2 vials (lyophilized)
Component C: Reaction Buffer	1 Vial (20 µL)