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Buccutite™ Rapid PerCP Antibody Labeling Kit
Production Scale Optimized for Labeling 1 mg Antibody Per Reaction
Buccutite™ Rapid PerCP Antibody Labeling Kits, designed for large-scale production, provide a streamlined approach for labeling antibodies with PerCP, APC, PE, and iFluor® tandem dyes. Compared to conventional protein-protein conjugation methods like the SMCC crosslinking technique, Buccutite™ conjugation is simple and more robust. Using a two-step mixing protocol, researchers can directly conjugate PerCP to any antibody or protein in less than 2 hours. Each Buccutite™ kit includes all the essential components for two labeling reactions and features a user-friendly, pre-packed spin column to maximize conjugate yield. Each Buccutite™ FOL-Activated PerCP vial provided in this kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it's important to remove stabilizing proteins like BSA from the sample and avoid using amine-rich buffers like Tris, which might disrupt the labeling process. PerCP is an intensely bright, red fluorescent phycobiliprotein with an excitation and emission maxima of ~477 nm and ~678 nm, respectively. With its large Stoke’s Shift and compatibility with blue laser excitation, PerCP forms an ideal combination with iFluor® 488 (or FITC) and PE for conducting one- to three-color analyses with just a single laser. PerCP conjugates are large complexes well-suited for cell surface labeling techniques, flow cytometry, spectral flow cytometry, and other immunoassays requiring high sensitivity but not photostability. With Buccuitte™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 5407 | 2 Labelings | Price |
Spectral properties
| Correction factor (280 nm) | 0.22 |
| Extinction coefficient (cm -1 M -1) | 406000 |
| Excitation (nm) | 477 |
| Emission (nm) | 678 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| UNSPSC | 12171501 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 18, 2025