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Buccutite™ Rapid Protein Crosslinking Kit
Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction
Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC. One end of the SMCC reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition it is quite difficult and tedious to quantify the number of maleimide groups on a protein. AAT Bioquest has developed a convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one protein, while FOL is added to another protein. Protein-protein cross-linking reaction is initiated by mixing Protein-1-Buccutite ™ MTA and Protein-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild neutral conditions without any catalyst required, and it is robust and efficient.
<p>Flow cytometry analysis of HL-60 cells stained with 1ug/ml Mouse IgG control (Green) or with 1ug/ml mouse Anti-Human HLA-ABC (W6/32 mAb)&nbsp; (Red) and then followed by Goat Anti-Mouse IgG-PE-Cy5 conjugate prepared with Buccutite&trade; Rapid PE-Cy5 Tandem Antibody Labeling Kit (Cat#1315).</p>
<p>Flow cytometry analysis of HL-60 cells stained with 1ug/ml Mouse IgG control (Green) or with 1ug/ml mouse Anti-Human HLA-ABC (W6/32 mAb)&nbsp; (Red) and then followed by Goat Anti-Mouse IgG-PE-Cy5 conjugate prepared with Buccutite&trade; Rapid PE-Cy5 Tandem Antibody Labeling Kit (Cat#1315).</p>
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13152 Labelings
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Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
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Page updated on October 8, 2025