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Buccutite™ Rapid trFluor™ D2 Acceptor Antibody Labeling Kit
Microscale Optimized for Labeling 100 ug Antibody Per Reaction
Many biological compounds present in cells, serum, or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences. Our trFluor™ Eu probes enable time-resolved fluorometry (TRF) for the assays that require high sensitivity. These trFluor™ Eu probes have large Stokes shifts and extremely long emission half-lives when compared to traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our trFluor™ Eu probes have relatively high stability, high emission yield, and the ability to be linked to biomolecules. Buccutite™ Rapid trFluor™ D2 Acceptor Antibody Labeling Kit provides a fast way to prepare the D2 acceptor-labeled bioconjugates that are used to pair to the trFluor™ Eu-labeled probes to develop TR-FRET assays.
D2 acceptor is used to pair with Eu-labeled probes (such as TR Fluor™ Eu) for developing TR-FRET assays. TR-FRET assays are much more sensitive than the regular FRET assays that suffer serve interference caused by the naturally fluorescent compounds present in cells, serum or other biological fluids. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences.
D2 acceptor is used to pair with Eu-labeled probes (such as TR Fluor™ Eu) for developing TR-FRET assays. TR-FRET assays are much more sensitive than the regular FRET assays that suffer serve interference caused by the naturally fluorescent compounds present in cells, serum or other biological fluids. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13022 Labelings
Price
 
Spectral properties
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on October 17, 2025