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C1-Bodi Fluor 500/510-C12 [equivalent to BODIPY® 500/510 C1, C12]

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Physical properties
Molecular weight404.30
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)512
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
404.30
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)
512
Emission (nm)
522
The green fluorescent BODIPY FL C12 fatty acid can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. BODIPY dyes are relatively environment insensitive and is fluorescent in both aqueous and lipid environments with sharp emission peak.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of C1-Bodi Fluor 500/510-C12 [equivalent to BODIPY® 500/510 C1, C12] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM247.341 µL1.237 mL2.473 mL12.367 mL24.734 mL
5 mM49.468 µL247.341 µL494.682 µL2.473 mL4.947 mL
10 mM24.734 µL123.671 µL247.341 µL1.237 mL2.473 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)90000
Excitation (nm)512
Emission (nm)522

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Citations


View all 1 citations: Citation Explorer
Anterior gradient 2 increases long-chain fatty acid uptake via stabilizing FABP1 and facilitates lipid accumulation
Authors: Wang, Yunqiu and Jia, Mengqi and Liang, Chuanjie and Sheng, Nan and Wang, Xiaodan and Wang, Fang and Luo, Yanhai and Jiang, Jin and Cai, Liangyu and Niu, Huanmin and others,
Journal: International journal of biological sciences (2021): 834

References


View all 8 references: Citation Explorer
Identification and biochemical characterization of five long-chain acyl-coenzyme A synthetases from the diatom Phaeodactylum tricornutum
Authors: Guo X, Jiang M, Wan X, Hu C, Gong Y.
Journal: Plant Physiol Biochem (2014): 33
Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish
Authors: Carten JD, Bradford MK, Farber SA.
Journal: Dev Biol (2011): 276
Adipose angiogenesis: quantitative methods to study microvessel growth, regression and remodeling in vivo
Authors: Xue Y, Lim S, Brakenhielm E, Cao Y.
Journal: Nat Protoc (2010): 912
The use of fluorescent fatty acid analogs as labels in trematode experimental infections
Authors: Keeney DB, Lagrue C, Bryan-Walker K, Khan N, Leung TL, Poulin R.
Journal: Exp Parasitol (2008): 15
High-throughput screening for fatty acid uptake inhibitors in humanized yeast identifies atypical antipsychotic drugs that cause dyslipidemias
Authors: Li H, Black PN, Chokshi A, S and oval-Alvarez A, Vatsyayan R, Sealls W, DiRusso CC.
Journal: J Lipid Res (2008): 230
A live-cell high-throughput screening assay for identification of fatty acid uptake inhibitors
Authors: Li H, Black PN, DiRusso CC.
Journal: Anal Biochem (2005): 11
A fluorescence-based high-performance liquid chromatographic assay to determine acid ceramidase activity
Authors: He X, Li CM, Park JH, Dagan A, Gatt S, Schuchman EH.
Journal: Anal Biochem (1999): 264
Disruption of the Saccharomyces cerevisiae homologue to the murine fatty acid transport protein impairs uptake and growth on long-chain fatty acids
Authors: Faergeman NJ, DiRusso CC, Elberger A, Knudsen J, Black PN.
Journal: J Biol Chem (1997): 8531