Cal-520®-Biocytin Conjugate

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	1341.55
Solvent	Water

Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

1341.55

Excitation (nm)

492

Emission (nm)

515

Quantum yield

0.75¹

Biotin and biocytin bind with high affinity to both avidins and streptavidins. Biotin and biocytin can be conjugated to many biomolecules without significantly altering the biological activity of the target molecules since and biocytin are relatively small molecules. Cal-520-biotin and Cal-520 biocytin conjugates have great calcium responses. Their avidin and streptavidin complexes have sensitive calcium responses. Conceivably Cal-520-biotin and Cal-520 biocytin conjugates could be bound to an IgG-avidin or an IgG-streptavidin conjugate for monitoring calcium change spatially for a specific target.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®-Biocytin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	74.541 µL	372.703 µL	745.406 µL	3.727 mL	7.454 mL
5 mM	14.908 µL	74.541 µL	149.081 µL	745.406 µL	1.491 mL
10 mM	7.454 µL	37.27 µL	74.541 µL	372.703 µL	745.406 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Cal-520®-Dextran Conjugate MW 3,000	492	515	0.75¹
Cal-520®-Dextran Conjugate MW 10,000	492	515	0.75¹
Cal-520®-Biotin Conjugate	492	515	0.75¹

Images

Figure 1. Chemical structure for Cal-520®-Biocytin Conjugate

Citations

View all 93 citations: Citation Explorer

Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157

High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging
Authors: Daily, Neil J and Du, Zhong-Wei and Wakatsuki, Tetsuro
Journal: ASSAY and Drug Development Technologies (2017)

HTS-Compatible Voltage-and Ca 2+-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems
Authors: Kettenhofen, Ralf
Journal: Stem Cell-Derived Models in Toxicology (2017): 135--152

Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes
Authors: Servin-Vences, M Rocio and Moroni, Mirko and Lewin, Gary R and Poole, Kate
Journal: eLife (2017): e21074

Synergistic drug combination effectively blocks Ebola virus infection
Authors: Sun, Wei and He, Shihua and Martínez-Romero, Carles and Kouznetsova, Jennifer and Tawa, Gregory and Xu, Miao and Shinn, Paul and Fisher, Ethan G and Long, Yan and Motabar, Omid and others, undefined
Journal: Antiviral Research (2017): 165--172

Properties of synchronous spontaneous Ca2+ transients in the mural cells of rat rectal arterioles
Authors: Mitsui, Retsu and Hashitani, Hikaru
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--14

A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse
Authors: Pan, Libiao and Yang, Junhua and Yang, Qian and Wang, Xiaomeng and Zhu, Liya and Liu, Yali and Lou, Huifang and Xu, Chou and Shen, Ying and Wang, Hao
Journal: Frontiers in molecular neuroscience (2017)

Retinal origin of direction selectivity in the superior colliculus
Authors: Shi, Xuefeng and Barchini, Jad and Ledesma, Hector Acaron and Koren, David and Jin, Yanjiao and Liu, Xiaorong and Wei, Wei and Cang, Jianhua
Journal: Nature Neuroscience (2017)

Bidirectional communication between sensory neurons and osteoblasts in an in vitro co-culture system
Authors: Kodama, Daisuke and Hirai, Takao and Kondo, Hisataka and Hamamura, Kazunori and Togari, Akifumi
Journal: FEBS Letters (2017)

Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools
Authors: Daily, Neil J and Santos, Radleigh and Vecchi, Joseph and Kemanli, Pinar and Wakatsuki, Tetsuro
Journal: Journal of evolving stem cell research (2017): 1