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Cal-520®-Biotin Conjugate

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Physical properties
Molecular weight1212.40
SolventWater
Spectral properties
Excitation (nm)492
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Show More (151)

OverviewpdfSDSpdfProtocol


Molecular weight
1212.40
Excitation (nm)
492
Emission (nm)
515
Quantum yield
0.751
Biotin and biocytin bind with high affinity to both avidins and streptavidins. Biotin and biocytin can be conjugated to many biomolecules without significantly altering the biological activity of the target molecules since and biocytin are relatively small molecules. Cal-520-biotin and Cal-520 biocytin conjugates have great calcium responses. Their avidin and streptavidin complexes have sensitive calcium responses. Conceivably Cal-520-biotin and Cal-520 biocytin conjugates could be bound to an IgG-avidin or an IgG-streptavidin conjugate for monitoring calcium change spatially for a specific target.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®-Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM82.481 µL412.405 µL824.81 µL4.124 mL8.248 mL
5 mM16.496 µL82.481 µL164.962 µL824.81 µL1.65 mL
10 mM8.248 µL41.241 µL82.481 µL412.405 µL824.81 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


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spectrum

Spectral properties

Excitation (nm)492
Emission (nm)515
Quantum yield0.751

Product Family


NameExcitation (nm)Emission (nm)Quantum yield
Cal-520®-Dextran Conjugate *MW 3,000*4925150.751
Cal-520®-Dextran Conjugate *MW 10,000*4925150.751
Cal-520®-Biocytin Conjugate4925150.751

Images


Citations


View all 93 citations: Citation Explorer
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging
Authors: Daily, Neil J and Du, Zhong-Wei and Wakatsuki, Tetsuro
Journal: ASSAY and Drug Development Technologies (2017)
HTS-Compatible Voltage-and Ca 2+-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems
Authors: Kettenhofen, Ralf
Journal: Stem Cell-Derived Models in Toxicology (2017): 135--152
Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes
Authors: Servin-Vences, M Rocio and Moroni, Mirko and Lewin, Gary R and Poole, Kate
Journal: eLife (2017): e21074
Synergistic drug combination effectively blocks Ebola virus infection
Authors: Sun, Wei and He, Shihua and Mart&iacute;nez-Romero, Carles and Kouznetsova, Jennifer and Tawa, Gregory and Xu, Miao and Shinn, Paul and Fisher, Ethan G and Long, Yan and Motabar, Omid and others, undefined
Journal: Antiviral Research (2017): 165--172
Properties of synchronous spontaneous Ca2+ transients in the mural cells of rat rectal arterioles
Authors: Mitsui, Retsu and Hashitani, Hikaru
Journal: Pfl&uuml;gers Archiv-European Journal of Physiology (2017): 1--14
A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse
Authors: Pan, Libiao and Yang, Junhua and Yang, Qian and Wang, Xiaomeng and Zhu, Liya and Liu, Yali and Lou, Huifang and Xu, Chou and Shen, Ying and Wang, Hao
Journal: Frontiers in molecular neuroscience (2017)
Retinal origin of direction selectivity in the superior colliculus
Authors: Shi, Xuefeng and Barchini, Jad and Ledesma, Hector Acaron and Koren, David and Jin, Yanjiao and Liu, Xiaorong and Wei, Wei and Cang, Jianhua
Journal: Nature Neuroscience (2017)
Bidirectional communication between sensory neurons and osteoblasts in an in vitro co-culture system
Authors: Kodama, Daisuke and Hirai, Takao and Kondo, Hisataka and Hamamura, Kazunori and Togari, Akifumi
Journal: FEBS Letters (2017)
Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools
Authors: Daily, Neil J and Santos, Radleigh and Vecchi, Joseph and Kemanli, Pinar and Wakatsuki, Tetsuro
Journal: Journal of evolving stem cell research (2017): 1