Name: Cal-520®-Biotin Conjugate
Brand: AAT Bioquest
SKU: 20605
Price: 348 USD
Availability: InStock

Cal-520®-Biotin Conjugate

Biotin and biocytin bind with high affinity to both avidins and streptavidins. Biotin and biocytin can be conjugated to many biomolecules without significantly altering the biological activity of the target molecules since and biocytin are relatively small molecules. Cal-520-biotin and Cal-520 biocytin conjugates have great calcium responses. Their avidin and streptavidin complexes have sensitive calcium responses. Conceivably Cal-520-biotin and Cal-520 biocytin conjugates could be bound to an IgG-avidin or an IgG-streptavidin conjugate for monitoring calcium change spatially for a specific target.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20605	5x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®-Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	82.481 µL	412.405 µL	824.81 µL	4.124 mL	8.248 mL
5 mM	16.496 µL	82.481 µL	164.962 µL	824.81 µL	1.65 mL
10 mM	8.248 µL	41.241 µL	82.481 µL	412.405 µL	824.81 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1212.4 g/mol	=	mL	x	mM	=	mmol

Citations

View all 93 citations: Citation Explorer

Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging

Authors:

Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud

Journal:

Proceedings of the IEEE (2017): 139--157

High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging

Authors:

Daily, Neil J and Du, Zhong-Wei and Wakatsuki, Tetsuro

Journal:

ASSAY and Drug Development Technologies (2017)

HTS-Compatible Voltage-and Ca 2+-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems

Authors:

Kettenhofen, Ralf

Journal:

Stem Cell-Derived Models in Toxicology (2017): 135--152

Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes

Authors:

Servin-Vences, M Rocio and Moroni, Mirko and Lewin, Gary R and Poole, Kate

Journal:

eLife (2017): e21074

Synergistic drug combination effectively blocks Ebola virus infection

Authors:

Sun, Wei and He, Shihua and Martínez-Romero, Carles and Kouznetsova, Jennifer and Tawa, Gregory and Xu, Miao and Shinn, Paul and Fisher, Ethan G and Long, Yan and Motabar, Omid and others, undefined

Journal:

Antiviral Research (2017): 165--172

Physical properties

Molecular weight	1212.40
Solvent	Water

Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025