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Cal-520®-Biotin Conjugate

Chemical structure for Cal-520®-Biotin Conjugate
Chemical structure for Cal-520®-Biotin Conjugate
Ordering information
Price ()
Catalog Number20605
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1212.40
Spectral properties
Excitation (nm)493
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Quantum yield
Biotin and biocytin bind with high affinity to both avidins and streptavidins. Biotin and biocytin can be conjugated to many biomolecules without significantly altering the biological activity of the target molecules since and biocytin are relatively small molecules. Cal-520-biotin and Cal-520 biocytin conjugates have great calcium responses. Their avidin and streptavidin complexes have sensitive calcium responses. Conceivably Cal-520-biotin and Cal-520 biocytin conjugates could be bound to an IgG-avidin or an IgG-streptavidin conjugate for monitoring calcium change spatially for a specific target.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®-Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM82.481 µL412.405 µL824.81 µL4.124 mL8.248 mL
5 mM16.496 µL82.481 µL164.962 µL824.81 µL1.65 mL
10 mM8.248 µL41.241 µL82.481 µL412.405 µL824.81 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectral properties

Excitation (nm)493
Emission (nm)515
Quantum yield0.751


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