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Cal-520®-Biotin Conjugate

Chemical structure for Cal-520®-Biotin Conjugate
Chemical structure for Cal-520®-Biotin Conjugate
Ordering information
Price ()
Catalog Number20605
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1212.40
SolventWater
Spectral properties
Excitation (nm)493
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
1212.40
Excitation (nm)
493
Emission (nm)
515
Quantum yield
0.751
Biotin and biocytin bind with high affinity to both avidins and streptavidins. Biotin and biocytin can be conjugated to many biomolecules without significantly altering the biological activity of the target molecules since and biocytin are relatively small molecules. Cal-520-biotin and Cal-520 biocytin conjugates have great calcium responses. Their avidin and streptavidin complexes have sensitive calcium responses. Conceivably Cal-520-biotin and Cal-520 biocytin conjugates could be bound to an IgG-avidin or an IgG-streptavidin conjugate for monitoring calcium change spatially for a specific target.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®-Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM82.481 µL412.405 µL824.81 µL4.124 mL8.248 mL
5 mM16.496 µL82.481 µL164.962 µL824.81 µL1.65 mL
10 mM8.248 µL41.241 µL82.481 µL412.405 µL824.81 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)493
Emission (nm)515
Quantum yield0.751

Citations


View all 93 citations: Citation Explorer
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators
Authors: Hashitani, Hikaru and Nguyen, Michael J and Noda, Haruka and Mitsui, Retsu and Higashi, Ryuhei and Ohta, Keisuke and Nakamura, Kei-Ichiro and Lang, Richard J
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--17
Extensive Ca 2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca 2+ hypersensitivity
Authors: Uehara, Akira and Murayama, Takashi and Yasukochi, Midori and Fill, Michael and Horie, Minoru and Okamoto, Toru and Matsuura, Yoshiharu and Uehara, Kiyoko and Fujimoto, Takahiro and Sakurai, Takashi and others, undefined
Journal: The Journal of general physiology (2017): 199--218
Synchronicity and Rhythmicity of Purkinje Cell Firing during Generalized Spike-and-Wave Discharges in a Natural Mouse Model of Absence Epilepsy Complex Spike Synchronicity during GSWDs
Authors: Kros, Lieke and Lindeman, S and er , undefined and Eelkman Rooda, Oscar HJ and Murugesan, Pavithra and Bina, Lorenzo and Bosman, Laurens WJ and De Zeeuw, Chris I and Hoebeek, Freek E
Journal: Frontiers in Cellular Neuroscience (2017): 346
Simultaneous Measurement of Neural Activities of Acute Mouse Hippocampal Slices Using Multi-Electrode Array System and Laser Confocal Calcium Imaging
Authors: Hamasaki, Yuuta and Haba, Natsumi and Iwata, Naoki and Uno, Yoshiki and Saito, Minoru
Journal: Journal of Behavioral and Brain Science (2017): 68
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone
Authors: Yu, Kanglun and Sellman, David P and Bahraini, Anoosh and Hagan, Mackenzie L and Elsherbini, Ahmed and Vanpelt, Kayce T and Marshall, Peyton L and Hamrick, Mark W and McNeil, Anna and McNeil, Paul L and others, undefined
Journal: Journal of Orthopaedic Research (2017)
Neonatal CX26 removal impairs neocortical development and leads to elevated anxiety
Authors: Su, Xin and Chen, Jing-Jing and Liu, Lin-Yun and Huang, Qian and Zhang, Li-Zhao and Li, Xiao-Yang and He, Xiang-Nan and Lu, Wenlian and Sun, Shan and Li, Huawei and others, undefined
Journal: Proceedings of the National Academy of Sciences (2017): 201613237
Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging
Authors: Li, YX and Gautam, V and Brüstle, A and Cockburn, IA and Daria, VR and Gillespie, C and Gaus, K and Alt, C and Lee, WM
Journal: Journal of Biophotonics (2017)
Mechanism for Triggered Waves in Atrial Myocytes
Authors: Shiferaw, Yohannes and Aistrup, Gary L and Wasserstrom, J Andrew
Journal: Biophysical Journal (2017): 656--670