Cal-520® alkyne

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	905.77
Solvent	DMSO

Spectral properties

Excitation (nm)	493
Emission (nm)	515
Quantum yield	0.75¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS

Molecular weight

905.77

Excitation (nm)

493

Emission (nm)

515

Quantum yield

0.75¹

Cal-520® alkyne is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to azido-containing biomolecules via the well-known click chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	110.403 µL	552.017 µL	1.104 mL	5.52 mL	11.04 mL
5 mM	22.081 µL	110.403 µL	220.807 µL	1.104 mL	2.208 mL
10 mM	11.04 µL	55.202 µL	110.403 µL	552.017 µL	1.104 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	493
Emission (nm)	515
Quantum yield	0.75¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Cal-520®, AM	493	515	0.75¹

Images

Figure 1. Cal-520 alkyne is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to azido-containing biomolecule via the well-known click chemistry.

References

View all 50 references: Citation Explorer

Simultaneous Acquisition of Mitochondrial Calcium Retention Capacity and Swelling to Measure Permeability Transition Sensitivity.
Authors: Mendoza, Arielys M and Karch, Jason
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 129-140

The microtubule network enables Src kinase interaction with the Na,K-ATPase to generate Ca2+ flashes in smooth muscle cells.
Authors: Rognant, Salomé and Kravtsova, Violetta V and Bouzinova, Elena V and Melnikova, Elizaveta V and Krivoi, Igor I and Pierre, Sandrine V and Aalkjaer, Christian and Jepps, Thomas A and Matchkov, Vladimir V
Journal: Frontiers in physiology (2022): 1007340

Effects of pyriproxyfen on zebrafish brain mitochondria and acetylcholinesterase.
Authors: Azevedo, Rafael D S and Falcão, Kivia V G and Assis, Caio R D and Martins, Regildo M G and Araújo, Marlyete C and Yogui, Gilvan T and Neves, Jorge L and Seabra, Gustavo M and Maia, Maria B S and Amaral, Ian P G and Leite, Ana C R and Bezerra, Ranilson S
Journal: Chemosphere (2021): 128029

Cationic Channel TRPV2 Overexpression Promotes Resistance to Cisplatin-Induced Apoptosis in Gastric Cancer Cells.
Authors: Laurino, Simona and Mazzone, Pellegrino and Ruggieri, Vitalba and Zoppoli, Pietro and Calice, Giovanni and Lapenta, Antonella and Ciuffi, Mario and Ignomirelli, Orazio and Vita, Giulia and Sgambato, Alessandro and Russi, Sabino and Falco, Geppino
Journal: Frontiers in pharmacology (2021): 746628

Assessment of Mitochondrial Ca2+ Uptake.
Authors: Deak, András T and Jean-Quartier, Claire and Bondarenko, Alexander I and Groschner, Lukas N and Malli, Roland and Graier, Wolfgang F and Waldeck-Weiermair, Markus
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 173-191

Analysis of Mitochondrial Calcium Retention Capacity in Cultured Cells: Permeabilized Cells Versus Isolated Mitochondria.
Authors: Jang, Sehwan and Chapa-Dubocq, Xavier R and Fossati, Silvia and Javadov, Sabzali
Journal: Frontiers in physiology (2021): 773839

The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca2+ Signaling Events During the Early Development of Zebrafish (Danio rerio).
Authors: Webb, Sarah E and Miller, Andrew L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 73-93

Mitochondria-coupled glucose phosphorylation develops after birth to modulate H2 O2 release and calcium handling in rat brain.
Authors: de-Souza-Ferreira, Eduardo and Rios-Neto, Izac Miranda and Martins, Eduarda Lopes and Galina, Antonio
Journal: Journal of neurochemistry (2019): 624-640

Ischemia time impacts on respiratory chain functions and Ca2+-handling of cardiac subsarcolemmal mitochondria subjected to ischemia reperfusion injury.
Authors: Leistner, Marcus and Sommer, Stefanie and Kanofsky, Peer and Leyh, Rainer and Sommer, Sebastian-Patrick
Journal: Journal of cardiothoracic surgery (2019): 92

Chloride channel blocker IAA-94 increases myocardial infarction by reducing calcium retention capacity of the cardiac mitochondria.
Authors: Ponnalagu, Devasena and Hussain, Ahmed Tafsirul and Thanawala, Rushi and Meka, Jahnavi and Bednarczyk, Piotr and Feng, Yansheng and Szewczyk, Adam and GururajaRao, Shubha and Bopassa, Jean C and Khan, Mahmood and Singh, Harpreet
Journal: Life sciences (2019): 116841