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Cal-520® alkyne
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Cal-520® alkyne is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to azido-containing biomolecules via the well-known click chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Cal-520®, AM | 492 | 515 | 0.751 |
References
View all 50 references: Citation Explorer
Simultaneous Acquisition of Mitochondrial Calcium Retention Capacity and Swelling to Measure Permeability Transition Sensitivity.
Authors: Mendoza, Arielys M and Karch, Jason
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 129-140
Authors: Mendoza, Arielys M and Karch, Jason
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 129-140
The microtubule network enables Src kinase interaction with the Na,K-ATPase to generate Ca2+ flashes in smooth muscle cells.
Authors: Rognant, Salomé and Kravtsova, Violetta V and Bouzinova, Elena V and Melnikova, Elizaveta V and Krivoi, Igor I and Pierre, Sandrine V and Aalkjaer, Christian and Jepps, Thomas A and Matchkov, Vladimir V
Journal: Frontiers in physiology (2022): 1007340
Authors: Rognant, Salomé and Kravtsova, Violetta V and Bouzinova, Elena V and Melnikova, Elizaveta V and Krivoi, Igor I and Pierre, Sandrine V and Aalkjaer, Christian and Jepps, Thomas A and Matchkov, Vladimir V
Journal: Frontiers in physiology (2022): 1007340
Effects of pyriproxyfen on zebrafish brain mitochondria and acetylcholinesterase.
Authors: Azevedo, Rafael D S and Falcão, Kivia V G and Assis, Caio R D and Martins, Regildo M G and Araújo, Marlyete C and Yogui, Gilvan T and Neves, Jorge L and Seabra, Gustavo M and Maia, Maria B S and Amaral, Ian P G and Leite, Ana C R and Bezerra, Ranilson S
Journal: Chemosphere (2021): 128029
Authors: Azevedo, Rafael D S and Falcão, Kivia V G and Assis, Caio R D and Martins, Regildo M G and Araújo, Marlyete C and Yogui, Gilvan T and Neves, Jorge L and Seabra, Gustavo M and Maia, Maria B S and Amaral, Ian P G and Leite, Ana C R and Bezerra, Ranilson S
Journal: Chemosphere (2021): 128029
Cationic Channel TRPV2 Overexpression Promotes Resistance to Cisplatin-Induced Apoptosis in Gastric Cancer Cells.
Authors: Laurino, Simona and Mazzone, Pellegrino and Ruggieri, Vitalba and Zoppoli, Pietro and Calice, Giovanni and Lapenta, Antonella and Ciuffi, Mario and Ignomirelli, Orazio and Vita, Giulia and Sgambato, Alessandro and Russi, Sabino and Falco, Geppino
Journal: Frontiers in pharmacology (2021): 746628
Authors: Laurino, Simona and Mazzone, Pellegrino and Ruggieri, Vitalba and Zoppoli, Pietro and Calice, Giovanni and Lapenta, Antonella and Ciuffi, Mario and Ignomirelli, Orazio and Vita, Giulia and Sgambato, Alessandro and Russi, Sabino and Falco, Geppino
Journal: Frontiers in pharmacology (2021): 746628
Assessment of Mitochondrial Ca2+ Uptake.
Authors: Deak, András T and Jean-Quartier, Claire and Bondarenko, Alexander I and Groschner, Lukas N and Malli, Roland and Graier, Wolfgang F and Waldeck-Weiermair, Markus
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 173-191
Authors: Deak, András T and Jean-Quartier, Claire and Bondarenko, Alexander I and Groschner, Lukas N and Malli, Roland and Graier, Wolfgang F and Waldeck-Weiermair, Markus
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 173-191