Name: Cal-520® azide
Brand: AAT Bioquest
SKU: 20613
Price: 465 USD
Availability: InStock

Cal-520® azide

Cal-520® azide is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be readily conjugated to alkyne-containing biomolecules via the well-known click chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20613	100 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	113.803 µL	569.016 µL	1.138 mL	5.69 mL	11.38 mL
5 mM	22.761 µL	113.803 µL	227.606 µL	1.138 mL	2.276 mL
10 mM	11.38 µL	56.902 µL	113.803 µL	569.016 µL	1.138 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	878.71 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Identifying subcutaneous tissue microcalcification by Fluo-3 AM imaging in cutaneous calciphylaxis.

Authors:

Zhu, Xiaodong and Liu, Yuqiu and Yang, Xin and Liu, Bicheng and Zhang, Xiaoliang

Journal:

Experimental dermatology (2022): 1632-1634

[The application of Fluo-3 AM in measurement of level of cytoplasmic calcium in thrombocytes by flow cytofluorometry].

Authors:

Petrischev, N N and Vasina, L V and Selyutin, A V and Chepanov, S V and Selkov, S A

Journal:

Klinicheskaia laboratornaia diagnostika (2017): 97-9

Basic methods for monitoring intracellular Ca2+ in cardiac myocytes using Fluo-3.

Authors:

Bito, Virginie and Sipido, Karin R and Macquaide, Niall

Journal:

Cold Spring Harbor protocols (2015): 392-7

[Application of the Ca2+ indicator fluo-3 and fluo-4 in the process of H2O2 induced apoptosis of A549 cell].

Authors:

Zhang, Siyang and Li, Chunyan and Gao, Jian and Qiu, Xueshan and Cui, Zeshi

Journal:

Zhongguo fei ai za zhi = Chinese journal of lung cancer (2014): 197-202

Temperature-induced labelling of Fluo-3 AM selectively yields brighter nucleus in adherent cells.

Authors:

Meng, Guixian and Pan, Leiting and Li, Cunbo and Hu, Fen and Shi, Xuechen and Lee, Imshik and Drevenšek-Olenik, Irena and Zhang, Xinzheng and Xu, Jingjun

Journal:

Biochemical and biophysical research communications (2014): 888-93

Physical properties

Molecular weight	878.71
Solvent	DMSO

Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 21, 2025