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Cal-520® azide
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Cal-520® azide is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be readily conjugated to alkyne-containing biomolecules via the well-known click chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Cal-520®, AM | 492 | 515 | 0.751 |
References
View all 50 references: Citation Explorer
Identifying subcutaneous tissue microcalcification by Fluo-3 AM imaging in cutaneous calciphylaxis.
Authors: Zhu, Xiaodong and Liu, Yuqiu and Yang, Xin and Liu, Bicheng and Zhang, Xiaoliang
Journal: Experimental dermatology (2022): 1632-1634
Authors: Zhu, Xiaodong and Liu, Yuqiu and Yang, Xin and Liu, Bicheng and Zhang, Xiaoliang
Journal: Experimental dermatology (2022): 1632-1634
[The application of Fluo-3 AM in measurement of level of cytoplasmic calcium in thrombocytes by flow cytofluorometry].
Authors: Petrischev, N N and Vasina, L V and Selyutin, A V and Chepanov, S V and Selkov, S A
Journal: Klinicheskaia laboratornaia diagnostika (2017): 97-9
Authors: Petrischev, N N and Vasina, L V and Selyutin, A V and Chepanov, S V and Selkov, S A
Journal: Klinicheskaia laboratornaia diagnostika (2017): 97-9
Basic methods for monitoring intracellular Ca2+ in cardiac myocytes using Fluo-3.
Authors: Bito, Virginie and Sipido, Karin R and Macquaide, Niall
Journal: Cold Spring Harbor protocols (2015): 392-7
Authors: Bito, Virginie and Sipido, Karin R and Macquaide, Niall
Journal: Cold Spring Harbor protocols (2015): 392-7
[Application of the Ca2+ indicator fluo-3 and fluo-4 in the process of H2O2 induced apoptosis of A549 cell].
Authors: Zhang, Siyang and Li, Chunyan and Gao, Jian and Qiu, Xueshan and Cui, Zeshi
Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer (2014): 197-202
Authors: Zhang, Siyang and Li, Chunyan and Gao, Jian and Qiu, Xueshan and Cui, Zeshi
Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer (2014): 197-202
Temperature-induced labelling of Fluo-3 AM selectively yields brighter nucleus in adherent cells.
Authors: Meng, Guixian and Pan, Leiting and Li, Cunbo and Hu, Fen and Shi, Xuechen and Lee, Imshik and Drevenšek-Olenik, Irena and Zhang, Xinzheng and Xu, Jingjun
Journal: Biochemical and biophysical research communications (2014): 888-93
Authors: Meng, Guixian and Pan, Leiting and Li, Cunbo and Hu, Fen and Shi, Xuechen and Lee, Imshik and Drevenšek-Olenik, Irena and Zhang, Xinzheng and Xu, Jingjun
Journal: Biochemical and biophysical research communications (2014): 888-93