Cal-520®-Dextran Conjugate *MW 10,000*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~11000 |
Solvent | Water |
Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
Cal-520®-Dextran Conjugate *MW 3,000* |
Overview | ![]() ![]() |
Molecular weight ~11000 | Excitation (nm) 493 | Emission (nm) 515 | Quantum yield 0.751 |
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.
Spectrum
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Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Product Family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-520®-Biotin Conjugate | 493 | 515 | 0.751 |
Cal-520®-Biocytin Conjugate | 493 | 515 | 0.751 |
Cal-590™-Dextran Conjugate *MW 3,000* | 563 | 584 | 0.621 |
Cal-590™-Dextran Conjugate *MW 10,000* | 563 | 584 | 0.621 |
Cal-630™-Dextran Conjugate *MW 3,000* | 609 | 626 | 0.371 |
Cal-630™-Dextran Conjugate *MW 10,000* | 609 | 626 | 0.371 |
Cal-670™-Dextran Conjugate *MW 3,000* | 667 | 680 | - |
Cal-670™-Dextran Conjugate *MW 10,000* | 667 | 680 | - |
Cal-770™-Dextran Conjugate *MW 3,000* | 758 | 783 | - |
Show More (2) |
Images
Citations
View all 103 citations: Citation Explorer
High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy
Authors: Meng, Guanghan and Zhang, Qinrong and Ji, Na
Journal: (2023): 293--329
Authors: Meng, Guanghan and Zhang, Qinrong and Ji, Na
Journal: (2023): 293--329
Neurophysiological functions and pharmacological tools of acidic and non-acidic Ca2+ stores
Authors: Martucci, Lora L and Cancela, Jos{\'e}-Manuel
Journal: Cell Calcium (2022): 102582
Authors: Martucci, Lora L and Cancela, Jos{\'e}-Manuel
Journal: Cell Calcium (2022): 102582
Network formation and reorganization of the olfactory system of Xenopus laevis
Authors: Hawkins, Sara Joy
Journal: (2022)
Authors: Hawkins, Sara Joy
Journal: (2022)
Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing
Authors: Koldaeva, Anzhelika and Zhang, Cary and Huang, Yu-Pei and Reinert, Janine Kristin and Mizuno, Seiya and Sugiyama, Fumihiro and Takahashi, Satoru and Soliman, Taha and Matsunami, Hiroaki and Fukunaga, Izumi
Journal: Journal of Neuroscience (2021): 6449--6467
Authors: Koldaeva, Anzhelika and Zhang, Cary and Huang, Yu-Pei and Reinert, Janine Kristin and Mizuno, Seiya and Sugiyama, Fumihiro and Takahashi, Satoru and Soliman, Taha and Matsunami, Hiroaki and Fukunaga, Izumi
Journal: Journal of Neuroscience (2021): 6449--6467
Astrocytic glutamate transporters reduce the neuronal and physiological influence of metabotropic glutamate receptors in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hermann, Gerlinda E and Hasser, Eileen M and Kline, David D
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2020): R545--R564
Authors: Martinez, Diana and Rogers, Richard C and Hermann, Gerlinda E and Hasser, Eileen M and Kline, David D
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2020): R545--R564
Reciprocal regulation of vacuolar calcium transport and V-ATPase activity, and the effects of Phosphatidylinositol 3, 5-bisphosphate
Authors: Miner, Gregory E and Rivera-Kohr, David A and Zhang, Chi and Sullivan, Katherine D and Guo, Annie and Fratti, Rutilio A
Journal: bioRxiv (2020)
Authors: Miner, Gregory E and Rivera-Kohr, David A and Zhang, Chi and Sullivan, Katherine D and Guo, Annie and Fratti, Rutilio A
Journal: bioRxiv (2020)
Phosphatidylinositol 3, 5-bisphosphate regulates Ca2+ transport during yeast vacuolar fusion through the Ca2+ ATPase Pmc1
Authors: Miner, Gregory E and Sullivan, Katherine D and Zhang, Chi and Rivera-Kohr, David and Guo, Annie and Hurst, Logan R and Ellis, Ez C and Starr, Matthew L and Jones, Brandon C and Fratti, Rutilio A
Journal: Traffic (2020): 503--517
Authors: Miner, Gregory E and Sullivan, Katherine D and Zhang, Chi and Rivera-Kohr, David and Guo, Annie and Hurst, Logan R and Ellis, Ez C and Starr, Matthew L and Jones, Brandon C and Fratti, Rutilio A
Journal: Traffic (2020): 503--517
Loss of excitatory amino acid transporter restraint following chronic intermittent hypoxia contributes to synaptic alterations in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hasser, Eileen M and Hermann, Gerlinda E and Kline, David D
Journal: Journal of Neurophysiology (2020): 2122--2135
Authors: Martinez, Diana and Rogers, Richard C and Hasser, Eileen M and Hermann, Gerlinda E and Kline, David D
Journal: Journal of Neurophysiology (2020): 2122--2135
50 Hz volumetric functional imaging with continuously adjustable depth of focus
Authors: Lu, Rongwen and Tanimoto, Masashi and Koyama, Minoru and Ji, Na
Journal: Biomedical Optics Express (2018): 1964--1976
Authors: Lu, Rongwen and Tanimoto, Masashi and Koyama, Minoru and Ji, Na
Journal: Biomedical Optics Express (2018): 1964--1976
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?