logo
AAT Bioquest

Cal-520®-Dextran Conjugate *MW 10,000*

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.

Spectrum

Citations

View all 106 citations: Citation Explorer
CalciSeg: A versatile approach for unsupervised segmentation of calcium imaging data
Authors: G{\"u}nzel, Yannick and Couzin-Fuchs, Einat and Paoli, Marco
Journal: NeuroImage (2024): 120758
SNMP1 is critical for sensitive detection of the desert locust aromatic courtship inhibition pheromone phenylacetonitrile
Authors: Lehmann, Joris and G{\"u}nzel, Yannick and Khosravian, Maryam and Cassau, Sina and Kraus, Susanne and Libnow, Johanna S and Chang, Hetan and Hansson, Bill S and Breer, Heinz and Couzin-Fuchs, Einat and others,
Journal: BMC biology (2024): 1--22
Ca2+-driven cytoplasmic backflow secures spindle position in fertilized mouse eggs
Authors: Totsuka, Takaya and Ohsugi, Miho
Journal: bioRxiv (2024): 2024--02
Neurophysiological functions and pharmacological tools of acidic and non-acidic Ca2+ stores
Authors: Martucci, Lora L and Cancela, Jos{\'e}-Manuel
Journal: Cell Calcium (2022): 102582
Page updated on October 11, 2024

Ordering information

Price
Unit size
Catalog Number20601
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

~11000

Solvent

Water

Spectral properties

Excitation (nm)

492

Emission (nm)

515

Quantum yield

0.751

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Fluorescence image of live Raw 264.7 cells stained with Cal-520&reg;-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite&trade; Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet&trade; LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence image of live Raw 264.7 cells stained with Cal-520&reg;-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite&trade; Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet&trade; LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence image of live Raw 264.7 cells stained with Cal-520&reg;-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite&trade; Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet&trade; LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence intensity increase of Cal-520&reg;-Dextran (Cat# 20600), Cal-520&reg;-Dextran (Cat# 20601) and Calcium-Green&trade;-1-Dextran upon binding saturated amount of calcium.