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Cal-520®-Dextran Conjugate *MW 10,000*

Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence intensity increase of Cal-520®-Dextran (Cat# 20600), Cal-520®-Dextran (Cat# 20601) and Calcium-Green™-1-Dextran upon binding saturated amount of calcium.
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Physical properties
Molecular weight~11000
SolventWater
Spectral properties
Excitation (nm)493
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


Molecular weight
~11000
Excitation (nm)
493
Emission (nm)
515
Quantum yield
0.751
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.

Spectrum


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spectrum

Spectral properties

Excitation (nm)493
Emission (nm)515
Quantum yield0.751

Images


Citations


View all 104 citations: Citation Explorer
Ca2+-driven cytoplasmic backflow secures spindle position in fertilized mouse eggs
Authors: Totsuka, Takaya and Ohsugi, Miho
Journal: bioRxiv (2024): 2024--02
Neurophysiological functions and pharmacological tools of acidic and non-acidic Ca2+ stores
Authors: Martucci, Lora L and Cancela, Jos{\'e}-Manuel
Journal: Cell Calcium (2022): 102582
Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing
Authors: Koldaeva, Anzhelika and Zhang, Cary and Huang, Yu-Pei and Reinert, Janine Kristin and Mizuno, Seiya and Sugiyama, Fumihiro and Takahashi, Satoru and Soliman, Taha and Matsunami, Hiroaki and Fukunaga, Izumi
Journal: Journal of Neuroscience (2021): 6449--6467
Astrocytic glutamate transporters reduce the neuronal and physiological influence of metabotropic glutamate receptors in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hermann, Gerlinda E and Hasser, Eileen M and Kline, David D
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2020): R545--R564
Reciprocal regulation of vacuolar calcium transport and V-ATPase activity, and the effects of Phosphatidylinositol 3, 5-bisphosphate
Authors: Miner, Gregory E and Rivera-Kohr, David A and Zhang, Chi and Sullivan, Katherine D and Guo, Annie and Fratti, Rutilio A
Journal: bioRxiv (2020)
Phosphatidylinositol 3, 5-bisphosphate regulates Ca2+ transport during yeast vacuolar fusion through the Ca2+ ATPase Pmc1
Authors: Miner, Gregory E and Sullivan, Katherine D and Zhang, Chi and Rivera-Kohr, David and Guo, Annie and Hurst, Logan R and Ellis, Ez C and Starr, Matthew L and Jones, Brandon C and Fratti, Rutilio A
Journal: Traffic (2020): 503--517
Loss of excitatory amino acid transporter restraint following chronic intermittent hypoxia contributes to synaptic alterations in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hasser, Eileen M and Hermann, Gerlinda E and Kline, David D
Journal: Journal of Neurophysiology (2020): 2122--2135
50 Hz volumetric functional imaging with continuously adjustable depth of focus
Authors: Lu, Rongwen and Tanimoto, Masashi and Koyama, Minoru and Ji, Na
Journal: Biomedical Optics Express (2018): 1964--1976