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Cal-520®-Dextran Conjugate *MW 10,000*

Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.
Fluorescence intensity increase of Cal-520®-Dextran (Cat# 20600), Cal-520®-Dextran (Cat# 20601) and Calcium-Green™-1-Dextran upon binding saturated amount of calcium.
Ordering information
Price ()
Catalog Number20601
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight11000
SolventWater
Spectral properties
Excitation (nm)493
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
11000
Excitation (nm)
493
Emission (nm)
515
Quantum yield
0.751
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®-Dextran Conjugate *MW 10,000* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM9.091 µL45.455 µL90.909 µL454.545 µL909.091 µL
5 mM1.818 µL9.091 µL18.182 µL90.909 µL181.818 µL
10 mM909.091 nL4.545 µL9.091 µL45.455 µL90.909 µL

Molarity calculator

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Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)493
Emission (nm)515
Quantum yield0.751

Citations


View all 100 citations: Citation Explorer
Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing
Authors: Koldaeva, Anzhelika and Zhang, Cary and Huang, Yu-Pei and Reinert, Janine Kristin and Mizuno, Seiya and Sugiyama, Fumihiro and Takahashi, Satoru and Soliman, Taha and Matsunami, Hiroaki and Fukunaga, Izumi
Journal: Journal of Neuroscience (2021): 6449--6467
Astrocytic glutamate transporters reduce the neuronal and physiological influence of metabotropic glutamate receptors in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hermann, Gerlinda E and Hasser, Eileen M and Kline, David D
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2020): R545--R564
Reciprocal regulation of vacuolar calcium transport and V-ATPase activity, and the effects of Phosphatidylinositol 3, 5-bisphosphate
Authors: Miner, Gregory E and Rivera-Kohr, David A and Zhang, Chi and Sullivan, Katherine D and Guo, Annie and Fratti, Rutilio A
Journal: bioRxiv (2020)
Phosphatidylinositol 3, 5-bisphosphate regulates Ca2+ transport during yeast vacuolar fusion through the Ca2+ ATPase Pmc1
Authors: Miner, Gregory E and Sullivan, Katherine D and Zhang, Chi and Rivera-Kohr, David and Guo, Annie and Hurst, Logan R and Ellis, Ez C and Starr, Matthew L and Jones, Brandon C and Fratti, Rutilio A
Journal: Traffic (2020): 503--517
Loss of excitatory amino acid transporter restraint following chronic intermittent hypoxia contributes to synaptic alterations in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hasser, Eileen M and Hermann, Gerlinda E and Kline, David D
Journal: Journal of Neurophysiology (2020): 2122--2135
50 Hz volumetric functional imaging with continuously adjustable depth of focus
Authors: Lu, Rongwen and Tanimoto, Masashi and Koyama, Minoru and Ji, Na
Journal: Biomedical Optics Express (2018): 1964--1976
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators
Authors: Hashitani, Hikaru and Nguyen, Michael J and Noda, Haruka and Mitsui, Retsu and Higashi, Ryuhei and Ohta, Keisuke and Nakamura, Kei-Ichiro and Lang, Richard J
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--17
Extensive Ca 2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca 2+ hypersensitivity
Authors: Uehara, Akira and Murayama, Takashi and Yasukochi, Midori and Fill, Michael and Horie, Minoru and Okamoto, Toru and Matsuura, Yoshiharu and Uehara, Kiyoko and Fujimoto, Takahiro and Sakurai, Takashi and others, undefined
Journal: The Journal of general physiology (2017): 199--218
Synchronicity and Rhythmicity of Purkinje Cell Firing during Generalized Spike-and-Wave Discharges in a Natural Mouse Model of Absence Epilepsy Complex Spike Synchronicity during GSWDs
Authors: Kros, Lieke and Lindeman, S and er , undefined and Eelkman Rooda, Oscar HJ and Murugesan, Pavithra and Bina, Lorenzo and Bosman, Laurens WJ and De Zeeuw, Chris I and Hoebeek, Freek E
Journal: Frontiers in Cellular Neuroscience (2017): 346