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Cal-520®, AM
Cal-520® AM is a cell-permeable calcium indicator with enhanced brightness and improved cellular retention compared to traditional indicators, providing robust fluorescence-based detection of intracellular calcium mobilization in live cells.
  • Significantly improved signal to noise (S/N) ratio
  • Excellent intracellular rentention, even without probenecid
  • Replaces outdated calcium indicators such as Fluo-3 and Fluo-4
ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-520™ AM (red curve), or Fluo-4 AM (blue curve) respectively with (left) or without probenecid (right) under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 µL per well in a Costar black wall/clear bottom 96-well plate. 100 µL of 5 µM Fluo-4 AM or Cal 520™ AM in HHBS (with or without probenecid) was added into the cells, and the cells were incubated at 37 °C for 1 hour. ATP (50 μL/well) was added using FlexSation to achieve the final indicated concentrations.
ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-520™ AM (red curve), or Fluo-4 AM (blue curve) respectively with (left) or without probenecid (right) under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 µL per well in a Costar black wall/clear bottom 96-well plate. 100 µL of 5 µM Fluo-4 AM or Cal 520™ AM in HHBS (with or without probenecid) was added into the cells, and the cells were incubated at 37 °C for 1 hour. ATP (50 μL/well) was added using FlexSation to achieve the final indicated concentrations.
protocol
Protocol
COA
CatalogSize
Price
Quantity
2113010x50 ug
Price
211311 mg
Price
 
Physical properties
Dissociation constant (Kd, nM)320
Molecular weight1102.95
SolventDMSO
Spectral properties
Excitation (nm)492
Emission (nm)515
Quantum yield
0.75
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC
EmissionFITC
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 9, 2025