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Cal-630™ AM
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. x-Rhod-1 is commonly used as a red fluorescent calcium indicator. However, x-Rhod-1 is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-630™ has been developed to improve x-Rhod-1 cell loading and calcium response while maintaining the spectral wavelength of x-Rhod-1, making it compatible with Texas Red® filter set. In CHO and HEK cells Cal-630™ AM has cellular calcium response that is much more sensitive than x-Rhod-1. The spectra of Cal-630 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.
ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-630™ AM (red curve). CHO-K1 cells were seeded overnight at 50,000 cells per 100 uL per well in a Costar black wall/clear bottom 96-well plate. 100 uL of 5 µM Cal-630 ™ AM in HHBS (with 1.0 mM probenecid) was added into the cells and incubated at 37 °C for 1 hour. ATP (50 uL/well) was added using FlexSation to achieve the final indicated concentrations.
ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-630™ AM (red curve). CHO-K1 cells were seeded overnight at 50,000 cells per 100 uL per well in a Costar black wall/clear bottom 96-well plate. 100 uL of 5 µM Cal-630 ™ AM in HHBS (with 1.0 mM probenecid) was added into the cells and incubated at 37 °C for 1 hour. ATP (50 uL/well) was added using FlexSation to achieve the final indicated concentrations.
protocol
Protocol
COA
CatalogSize
Price
Quantity
205305x50 ug
Price
2053110x50 ug
Price
205321 mg
Price
 
Physical properties
Dissociation constant (Kd, nM)792
Molecular weight1282.89
SolventDMSO
Spectral properties
Excitation (nm)609
Emission (nm)626
Quantum yield
0.37
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationTexas Red
EmissionTexas Red
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation600
Emission640
Cutoff630
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 14, 2025