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Cal-520® amine

Cal-520® amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Quantum yield
Cal-520®, AM4925150.751

References

View all 50 references: Citation Explorer
Oligosaccharide Blocks PAR1 (Proteinase-Activated Receptor 1)-PAR4-Mediated Platelet Activation by Binding to Thrombin Exosite II and Impairs Thrombosis.
Authors: Li, Sujuan and Wang, Weili and Lin, Lisha and Yang, Lian and Cai, Ying and Yang, Xingzhi and Zhang, Taocui and Xiao, Chuang and Yan, Hui and Gao, Na and Zhao, Jinhua
Journal: Arteriosclerosis, thrombosis, and vascular biology (2023): 253-266
The Calcium Transient Coupled to the L-Type Calcium Current Attenuates Action Potential Alternans.
Authors: Warren, Mark and Poelzing, Steven
Journal: bioRxiv : the preprint server for biology (2023)
Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer.
Authors: Romshin, Alexey M and Zeeb, Vadim and Glushkov, Evgenii and Radenovic, Aleksandra and Sinogeikin, Andrey G and Vlasov, Igor I
Journal: Scientific reports (2023): 8546
Bipartite Activation of Sensory Neurons by a TRPA1 Agonist Allyl Isothiocyanate Is Reflected by Complex Ca2+ Influx and CGRP Release Patterns: Enhancement by NGF and Inhibition with VAMP and SNAP-25 Cleaving Botulinum Neurotoxins.
Authors: Belinskaia, Mariia and Wang, Jiafu and Kaza, Seshu Kumar and Antoniazzi, Caren and Zurawski, Tomas and Dolly, J Oliver and Lawrence, Gary W
Journal: International journal of molecular sciences (2023)
FM1-43 Dye Memorizes Piezo1 Activation in the Trigeminal Nociceptive System Implicated in Migraine Pain.
Authors: Della Pietra, Adriana and Mikhailov, Nikita and Giniatullin, Rashid
Journal: International journal of molecular sciences (2023)
Page updated on December 11, 2024

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Catalog Number20612
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Physical properties

Molecular weight

909.68

Solvent

DMSO

Spectral properties

Excitation (nm)

492

Emission (nm)

515

Quantum yield

0.751

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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