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Cal-520® amine

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Physical properties
Molecular weight909.68
Spectral properties
Excitation (nm)492
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Excitation (nm)
Emission (nm)
Quantum yield
Cal-520® amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM109.929 µL549.644 µL1.099 mL5.496 mL10.993 mL
5 mM21.986 µL109.929 µL219.858 µL1.099 mL2.199 mL
10 mM10.993 µL54.964 µL109.929 µL549.644 µL1.099 mL

Molarity calculator

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Spectral properties

Excitation (nm)492
Emission (nm)515
Quantum yield0.751

Product Family

NameExcitation (nm)Emission (nm)Quantum yield
Cal-520®, AM4925150.751



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