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Cal-520® amine

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Physical properties
Molecular weight909.68
SolventDMSO
Spectral properties
Excitation (nm)492
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (78)

OverviewpdfSDSpdfProtocol


Molecular weight
909.68
Excitation (nm)
492
Emission (nm)
515
Quantum yield
0.751
Cal-520® amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM109.929 µL549.644 µL1.099 mL5.496 mL10.993 mL
5 mM21.986 µL109.929 µL219.858 µL1.099 mL2.199 mL
10 mM10.993 µL54.964 µL109.929 µL549.644 µL1.099 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)492
Emission (nm)515
Quantum yield0.751

Product Family


NameExcitation (nm)Emission (nm)Quantum yield
Cal-520®, AM4925150.751

Images


References


View all 50 references: Citation Explorer
Oligosaccharide Blocks PAR1 (Proteinase-Activated Receptor 1)-PAR4-Mediated Platelet Activation by Binding to Thrombin Exosite II and Impairs Thrombosis.
Authors: Li, Sujuan and Wang, Weili and Lin, Lisha and Yang, Lian and Cai, Ying and Yang, Xingzhi and Zhang, Taocui and Xiao, Chuang and Yan, Hui and Gao, Na and Zhao, Jinhua
Journal: Arteriosclerosis, thrombosis, and vascular biology (2023): 253-266
The Calcium Transient Coupled to the L-Type Calcium Current Attenuates Action Potential Alternans.
Authors: Warren, Mark and Poelzing, Steven
Journal: bioRxiv : the preprint server for biology (2023)
Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer.
Authors: Romshin, Alexey M and Zeeb, Vadim and Glushkov, Evgenii and Radenovic, Aleksandra and Sinogeikin, Andrey G and Vlasov, Igor I
Journal: Scientific reports (2023): 8546
Bipartite Activation of Sensory Neurons by a TRPA1 Agonist Allyl Isothiocyanate Is Reflected by Complex Ca2+ Influx and CGRP Release Patterns: Enhancement by NGF and Inhibition with VAMP and SNAP-25 Cleaving Botulinum Neurotoxins.
Authors: Belinskaia, Mariia and Wang, Jiafu and Kaza, Seshu Kumar and Antoniazzi, Caren and Zurawski, Tomas and Dolly, J Oliver and Lawrence, Gary W
Journal: International journal of molecular sciences (2023)
FM1-43 Dye Memorizes Piezo1 Activation in the Trigeminal Nociceptive System Implicated in Migraine Pain.
Authors: Della Pietra, Adriana and Mikhailov, Nikita and Giniatullin, Rashid
Journal: International journal of molecular sciences (2023)
BIN1, Myotubularin, and Dynamin-2 Coordinate T-Tubule Growth in Cardiomyocytes.
Authors: Perdreau-Dahl, Harmonie and Lipsett, David B and Frisk, Michael and Kermani, Fatemeh and Carlson, Cathrine R and Brech, Andreas and Shen, Xin and Bergan-Dahl, Anna and Hou, Yufeng and Tuomainen, Tomi and Tavi, Pasi and Jones, Peter P and Lunde, Marianne and Wasserstrom, J Andrew and Laporte, Jocelyn and Ullrich, Nina D and Christensen, Geir and Morth, J Preben and Louch, William E
Journal: Circulation research (2023): e188-e205
Capsazepine antagonizes TRPV1 activation induced by thermal and osmotic stimuli in human odontoblast-like cells.
Authors: Bernal-Cepeda, Lilia Jadith and Velandia-Romero, Myriam L and Castellanos, Jaime E
Journal: Journal of oral biology and craniofacial research (2023): 71-77
Vascular Smooth Muscle TRPV4 (Transient Receptor Potential Vanilloid Family Member 4) Channels Regulate Vasoconstriction and Blood Pressure in Obesity.
Authors: Zhu, Yifei and Chu, Yuan and Wang, Sheng and Tang, Junjian and Li, Hu and Feng, Lei and Yu, Fan and Ma, Xin
Journal: Hypertension (Dallas, Tex. : 1979) (2023): 757-770
Effects of apocynin on ISO-induced delayed afterdepolarizations in rat atrial myocytes and the underlying mechanisms.
Authors: Zhang, Nan and Dan, Qing and Dong, Ying and Liu, Yan and Zhuang, Wenjuan and Xie, Zhonghui and Zhao, Qianqian and Lin, Kun and Li, Yang
Journal: Biochemical and biophysical research communications (2023): 36-42
The Mechanism of the Anti-Cardiac Hypertrophy Effect of Glycyrrhizic Acid Is Related to Reducing STIM1-Dependent Store-Operated Calcium Entry.
Authors: Cheng, X and Huang, L
Journal: Bulletin of experimental biology and medicine (2023): 701-706