Cal-520® amine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 909.68 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 493 |
| Emission (nm) | 515 |
| Quantum yield | 0.751 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| Cal-520® maleimide |
| Cal-520® azide |
| Cal-520® alkyne |
| Overview |  SDSProtocol |
Molecular weight 909.68 | Excitation (nm) 493 | Emission (nm) 515 | Quantum yield 0.751 |
Cal-520® amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 109.929 µL | 549.644 µL | 1.099 mL | 5.496 mL | 10.993 mL |
| 5 mM | 21.986 µL | 109.929 µL | 219.858 µL | 1.099 mL | 2.199 mL |
| 10 mM | 10.993 µL | 54.964 µL | 109.929 µL | 549.644 µL | 1.099 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Excitation (nm) | 493 |
| Emission (nm) | 515 |
| Quantum yield | 0.751 |
Images
Figure 1. Cal-520 amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry.
References
View all 50 references: Citation Explorer
Metformin reverses oxidative stress‑induced mitochondrial dysfunction in pre‑osteoblasts via the EGFR/GSK‑3β/calcium pathway.
Authors: Cao, Fangming and Yang, Keda and Qiu, Shui and Li, Jie and Jiang, Wen and Tao, Lin and Zhu, Yue
Journal: International journal of molecular medicine (2023)
Authors: Cao, Fangming and Yang, Keda and Qiu, Shui and Li, Jie and Jiang, Wen and Tao, Lin and Zhu, Yue
Journal: International journal of molecular medicine (2023)
Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells.
Authors: Jiang, Man and Zhang, Yi-Xin and Bu, Wen-Jie and Li, Ping and Chen, Jia-Hui and Cao, Ming and Dong, Yan-Chao and Sun, Zhi-Jie and Dong, De-Li
Journal: British journal of pharmacology (2023): 1862-1877
Authors: Jiang, Man and Zhang, Yi-Xin and Bu, Wen-Jie and Li, Ping and Chen, Jia-Hui and Cao, Ming and Dong, Yan-Chao and Sun, Zhi-Jie and Dong, De-Li
Journal: British journal of pharmacology (2023): 1862-1877
Pterosin sesquiterpenoids from Pteris laeta Wall. ex Ettingsh. protect cells from glutamate excitotoxicity by modulating mitochondrial signals.
Authors: Cheng, Aifang and Zhang, Yan and Sun, Jin and Huang, Duli and Sulaiman, Jordy Evan and Huang, Xin and Wu, Long and Ye, Wenkang and Wu, Chuanhai and Lam, Henry and Shi, Yusheng and Qian, Pei-Yuan
Journal: Journal of ethnopharmacology (2023): 116308
Authors: Cheng, Aifang and Zhang, Yan and Sun, Jin and Huang, Duli and Sulaiman, Jordy Evan and Huang, Xin and Wu, Long and Ye, Wenkang and Wu, Chuanhai and Lam, Henry and Shi, Yusheng and Qian, Pei-Yuan
Journal: Journal of ethnopharmacology (2023): 116308
Live Imaging of CAR T Cell Ca2+ Signals in Tumor Slices Using Confocal Microscopy.
Authors: Espie, David and Barrin, Sarah and Rajnpreht, Irena and Vimeux, Lene and Donnadieu, Emmanuel
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 453-462
Authors: Espie, David and Barrin, Sarah and Rajnpreht, Irena and Vimeux, Lene and Donnadieu, Emmanuel
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 453-462
Aluminum alters excitability by inhibiting calcium, sodium, and potassium currents in bovine chromaffin cells.
Authors: Baraibar, Andrés M and de Pascual, Ricardo and Carretero, Victoria Jiménez and Liccardi, Ninfa and Juárez, Natalia Hernández and Hernández-Guijo, Jesús M
Journal: Journal of neurochemistry (2023): 162-176
Authors: Baraibar, Andrés M and de Pascual, Ricardo and Carretero, Victoria Jiménez and Liccardi, Ninfa and Juárez, Natalia Hernández and Hernández-Guijo, Jesús M
Journal: Journal of neurochemistry (2023): 162-176
Dibazol-induced relaxation of ophthalmic artery in C57BL/6J mice is correlated with the potency to inhibit voltage-gated Ca2+ channels.
Authors: Xu, Xinrong and Hou, Xiaomin and Xing, Ye and Feng, Tianfeng and Chai, Lina and Guo, Yunting and Chen, Liangjing and Shi, Yiwei and Qin, Xiaojiang
Journal: Experimental eye research (2023): 109468
Authors: Xu, Xinrong and Hou, Xiaomin and Xing, Ye and Feng, Tianfeng and Chai, Lina and Guo, Yunting and Chen, Liangjing and Shi, Yiwei and Qin, Xiaojiang
Journal: Experimental eye research (2023): 109468
Calcium Imaging of Non-adherent Cells.
Authors: Bye, Lydia J and Finol-Urdaneta, Rocio K and Adams, David J
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 361-369
Authors: Bye, Lydia J and Finol-Urdaneta, Rocio K and Adams, David J
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 361-369
Comparing the effects of chemical Ca2+ dyes and R-GECO on contractility and Ca2+ transients in adult and human iPSC cardiomyocytes.
Authors: Robinson, Paul and Sparrow, Alexander J and Psaras, Yiangos and Steeples, Violetta and Simon, Jillian N and Broyles, Connor N and Chang, Yu-Fen and Brook, Frances A and Wang, Ying-Jie and Blease, Andrew and Zhang, Xiaoyu and Abassi, Yama A and Geeves, Michael A and Toepfer, Christopher N and Watkins, Hugh and Redwood, Charles and Daniels, Matthew J
Journal: Journal of molecular and cellular cardiology (2023): 44-57
Authors: Robinson, Paul and Sparrow, Alexander J and Psaras, Yiangos and Steeples, Violetta and Simon, Jillian N and Broyles, Connor N and Chang, Yu-Fen and Brook, Frances A and Wang, Ying-Jie and Blease, Andrew and Zhang, Xiaoyu and Abassi, Yama A and Geeves, Michael A and Toepfer, Christopher N and Watkins, Hugh and Redwood, Charles and Daniels, Matthew J
Journal: Journal of molecular and cellular cardiology (2023): 44-57
C-subfamily ATP binding cassette transporters extrude the calcium fluorescent probe fluo-4 from a cone photoreceptor cell line.
Authors: García-de-Diego, Antonio-Miguel
Journal: Naunyn-Schmiedeberg's archives of pharmacology (2023)
Authors: García-de-Diego, Antonio-Miguel
Journal: Naunyn-Schmiedeberg's archives of pharmacology (2023)
TRPA1 promotes the maturation of embryonic stem cell-derived cardiomyocytes by regulating mitochondrial biogenesis and dynamics.
Authors: Ding, Qianqian and Liu, Xianji and Qi, Yanxiang and Yao, Xiaoqiang and Tsang, Suk Ying
Journal: Stem cell research & therapy (2023): 158
Authors: Ding, Qianqian and Liu, Xianji and Qi, Yanxiang and Yao, Xiaoqiang and Tsang, Suk Ying
Journal: Stem cell research & therapy (2023): 158