Cal-520®-Dextran Conjugate *MW 10,000*
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.
![Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcal-520-dextran-conjugate-mw-10-000%2Ffigure-for-cal-520-dextran-conjugate-mw-10-000_vG9qR.jpg&w=640&q=75)
![Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcal-520-dextran-conjugate-mw-10-000%2Ffigure-for-cal-520-dextran-conjugate-mw-10-000_vG9qR.jpg&w=640&q=75)
![Fluorescence image of live Raw 264.7 cells stained with Cal-520®-Dextran Conjugate MW 10,000 (Cat # 20601, Green) to visualize lysosomal Ca2+ , LysoBrite™ Red (Cat # 22645, Red) to visualize lysosomes and Nuclear Violet™ LCS1 (Cat# 17543, Blue) to visualize nuclei.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcal-520-dextran-conjugate-mw-10-000%2Ffigure-for-cal-520-dextran-conjugate-mw-10-000_vG9qR.jpg&w=128&q=25)
![Fluorescence intensity increase of Cal-520®-Dextran (Cat# 20600), Cal-520®-Dextran (Cat# 20601) and Calcium-Green™-1-Dextran upon binding saturated amount of calcium.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcal-520-dextran-conjugate-mw-10-000%2Ffigure-for-cal-520-dextran-conjugate-mw-10-000_KzSw5.jpg&w=128&q=25)
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-520®-Biotin Conjugate | 492 | 515 | 0.751 |
Cal-520®-Biocytin Conjugate | 492 | 515 | 0.751 |
Cal-590™-Dextran Conjugate *MW 3,000* | 563 | 584 | 0.621 |
Cal-590™-Dextran Conjugate *MW 10,000* | 563 | 584 | 0.621 |
Cal-630™-Dextran Conjugate *MW 3,000* | 609 | 626 | 0.371 |
Cal-630™-Dextran Conjugate *MW 10,000* | 609 | 626 | 0.371 |
Cal-670™-Dextran Conjugate *MW 3,000* | 667 | 680 | - |
Cal-670™-Dextran Conjugate *MW 10,000* | 667 | 680 | - |
Cal-770™-Dextran Conjugate *MW 3,000* | 758 | 783 | - |
Show More (2) |
Citations
View all 105 citations: Citation Explorer
SNMP1 is critical for sensitive detection of the desert locust aromatic courtship inhibition pheromone phenylacetonitrile
Authors: Lehmann, Joris and G{\"u}nzel, Yannick and Khosravian, Maryam and Cassau, Sina and Kraus, Susanne and Libnow, Johanna S and Chang, Hetan and Hansson, Bill S and Breer, Heinz and Couzin-Fuchs, Einat and others,
Journal: BMC biology (2024): 1--22
Authors: Lehmann, Joris and G{\"u}nzel, Yannick and Khosravian, Maryam and Cassau, Sina and Kraus, Susanne and Libnow, Johanna S and Chang, Hetan and Hansson, Bill S and Breer, Heinz and Couzin-Fuchs, Einat and others,
Journal: BMC biology (2024): 1--22
Ca2+-driven cytoplasmic backflow secures spindle position in fertilized mouse eggs
Authors: Totsuka, Takaya and Ohsugi, Miho
Journal: bioRxiv (2024): 2024--02
Authors: Totsuka, Takaya and Ohsugi, Miho
Journal: bioRxiv (2024): 2024--02
High-Speed Neural Imaging with Synaptic Resolution: Bessel Focus Scanning Two-Photon Microscopy and Optical-Sectioning Widefield Microscopy
Authors: Meng, Guanghan and Zhang, Qinrong and Ji, Na
Journal: (2023): 293--329
Authors: Meng, Guanghan and Zhang, Qinrong and Ji, Na
Journal: (2023): 293--329
Neurophysiological functions and pharmacological tools of acidic and non-acidic Ca2+ stores
Authors: Martucci, Lora L and Cancela, Jos{\'e}-Manuel
Journal: Cell Calcium (2022): 102582
Authors: Martucci, Lora L and Cancela, Jos{\'e}-Manuel
Journal: Cell Calcium (2022): 102582
Network formation and reorganization of the olfactory system of Xenopus laevis
Authors: Hawkins, Sara Joy
Journal: (2022)
Authors: Hawkins, Sara Joy
Journal: (2022)