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Cal-520®-Dextran Conjugate *MW 3,000*

Fluorescence intensity increase of Cal-520®-Dextran (Cat# 20600), Cal-520®-Dextran (Cat# 20601) and Calcium-Green™-1-Dextran upon binding saturated amount of calcium.
Fluorescence intensity increase of Cal-520®-Dextran (Cat# 20600), Cal-520®-Dextran (Cat# 20601) and Calcium-Green™-1-Dextran upon binding saturated amount of calcium.
Fluorescence intensity increase of Cal-520®-Dextran (Cat# 20600), Cal-520®-Dextran (Cat# 20601) and Calcium-Green™-1-Dextran upon binding saturated amount of calcium.
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Physical properties
Molecular weight~4000
SolventWater
Spectral properties
Excitation (nm)492
Emission (nm)515
Quantum yield0.751
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


Molecular weight
~4000
Excitation (nm)
492
Emission (nm)
515
Quantum yield
0.751
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. Compared to Oregon Green BAPTA-1 dextran, Cal-520 dextran exhibits much lower background and much larger calcium-induced fluorescence enhancement.

Spectrum


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spectrum

Spectral properties

Excitation (nm)492
Emission (nm)515
Quantum yield0.751

Images


Citations


View all 99 citations: Citation Explorer
Astrocytic glutamate transporters reduce the neuronal and physiological influence of metabotropic glutamate receptors in nucleus tractus solitarii
Authors: Martinez, Diana and Rogers, Richard C and Hermann, Gerlinda E and Hasser, Eileen M and Kline, David D
Journal: American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (2020): R545--R564
Phosphatidylinositol 3, 5-Bisphosphate regulates Ca2+ Transport During Yeast Vacuolar Fusion through Activation of the Ca2+ ATPase Pmc1
Authors: Fratti, Rutilio A and Miner, Gregory E and Sullivan, Katherine D and Guo, Annie and Starr, Matthew L and Ellis, Ez and Jones, Br and on, undefined
Journal: bioRxiv (2019): 533067
Response patterns and neurogenesis of the olfactory bulb of Xenopus laevis during metamorphosis
Authors: ARIAS, PAOLA LUZ ERENDIDA SEGOVIANO and others,
Journal: (2018)
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators
Authors: Hashitani, Hikaru and Nguyen, Michael J and Noda, Haruka and Mitsui, Retsu and Higashi, Ryuhei and Ohta, Keisuke and Nakamura, Kei-Ichiro and Lang, Richard J
Journal: Pfl&uuml;gers Archiv-European Journal of Physiology (2017): 1--17
Extensive Ca 2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca 2+ hypersensitivity
Authors: Uehara, Akira and Murayama, Takashi and Yasukochi, Midori and Fill, Michael and Horie, Minoru and Okamoto, Toru and Matsuura, Yoshiharu and Uehara, Kiyoko and Fujimoto, Takahiro and Sakurai, Takashi and others, undefined
Journal: The Journal of general physiology (2017): 199--218
Synchronicity and Rhythmicity of Purkinje Cell Firing during Generalized Spike-and-Wave Discharges in a Natural Mouse Model of Absence Epilepsy Complex Spike Synchronicity during GSWDs
Authors: Kros, Lieke and Lindeman, S and er , undefined and Eelkman Rooda, Oscar HJ and Murugesan, Pavithra and Bina, Lorenzo and Bosman, Laurens WJ and De Zeeuw, Chris I and Hoebeek, Freek E
Journal: Frontiers in Cellular Neuroscience (2017): 346
Simultaneous Measurement of Neural Activities of Acute Mouse Hippocampal Slices Using Multi-Electrode Array System and Laser Confocal Calcium Imaging
Authors: Hamasaki, Yuuta and Haba, Natsumi and Iwata, Naoki and Uno, Yoshiki and Saito, Minoru
Journal: Journal of Behavioral and Brain Science (2017): 68