Cal-520® NHS Ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1048.9 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Calcium Indicators, Chemical Reagents, Dyes by Functional Group, Intracellular Ions, Physiological Probes
Molecular weight 1048.9 | Excitation (nm) 493 | Emission (nm) 515 | Quantum yield 0.751 |
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cal-520 has been proven to be the best green fluorescent calcium indicator. Conceivably this amine-reactive Cal-520 NHS ester could be coupled to an IgG or other carrier molecules to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 95.338 µL | 476.69 µL | 953.38 µL | 4.767 mL | 9.534 mL |
5 mM | 19.068 µL | 95.338 µL | 190.676 µL | 953.38 µL | 1.907 mL |
10 mM | 9.534 µL | 47.669 µL | 95.338 µL | 476.69 µL | 953.38 µL |
Molarity calculator
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Spectrum
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Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Citations
View all 93 citations: Citation Explorer
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators
Authors: Hashitani, Hikaru and Nguyen, Michael J and Noda, Haruka and Mitsui, Retsu and Higashi, Ryuhei and Ohta, Keisuke and Nakamura, Kei-Ichiro and Lang, Richard J
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--17
Authors: Hashitani, Hikaru and Nguyen, Michael J and Noda, Haruka and Mitsui, Retsu and Higashi, Ryuhei and Ohta, Keisuke and Nakamura, Kei-Ichiro and Lang, Richard J
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--17
Extensive Ca 2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca 2+ hypersensitivity
Authors: Uehara, Akira and Murayama, Takashi and Yasukochi, Midori and Fill, Michael and Horie, Minoru and Okamoto, Toru and Matsuura, Yoshiharu and Uehara, Kiyoko and Fujimoto, Takahiro and Sakurai, Takashi and others, undefined
Journal: The Journal of general physiology (2017): 199--218
Authors: Uehara, Akira and Murayama, Takashi and Yasukochi, Midori and Fill, Michael and Horie, Minoru and Okamoto, Toru and Matsuura, Yoshiharu and Uehara, Kiyoko and Fujimoto, Takahiro and Sakurai, Takashi and others, undefined
Journal: The Journal of general physiology (2017): 199--218
Synchronicity and Rhythmicity of Purkinje Cell Firing during Generalized Spike-and-Wave Discharges in a Natural Mouse Model of Absence Epilepsy Complex Spike Synchronicity during GSWDs
Authors: Kros, Lieke and Lindeman, S and er , undefined and Eelkman Rooda, Oscar HJ and Murugesan, Pavithra and Bina, Lorenzo and Bosman, Laurens WJ and De Zeeuw, Chris I and Hoebeek, Freek E
Journal: Frontiers in Cellular Neuroscience (2017): 346
Authors: Kros, Lieke and Lindeman, S and er , undefined and Eelkman Rooda, Oscar HJ and Murugesan, Pavithra and Bina, Lorenzo and Bosman, Laurens WJ and De Zeeuw, Chris I and Hoebeek, Freek E
Journal: Frontiers in Cellular Neuroscience (2017): 346
Simultaneous Measurement of Neural Activities of Acute Mouse Hippocampal Slices Using Multi-Electrode Array System and Laser Confocal Calcium Imaging
Authors: Hamasaki, Yuuta and Haba, Natsumi and Iwata, Naoki and Uno, Yoshiki and Saito, Minoru
Journal: Journal of Behavioral and Brain Science (2017): 68
Authors: Hamasaki, Yuuta and Haba, Natsumi and Iwata, Naoki and Uno, Yoshiki and Saito, Minoru
Journal: Journal of Behavioral and Brain Science (2017): 68
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone
Authors: Yu, Kanglun and Sellman, David P and Bahraini, Anoosh and Hagan, Mackenzie L and Elsherbini, Ahmed and Vanpelt, Kayce T and Marshall, Peyton L and Hamrick, Mark W and McNeil, Anna and McNeil, Paul L and others, undefined
Journal: Journal of Orthopaedic Research (2017)
Authors: Yu, Kanglun and Sellman, David P and Bahraini, Anoosh and Hagan, Mackenzie L and Elsherbini, Ahmed and Vanpelt, Kayce T and Marshall, Peyton L and Hamrick, Mark W and McNeil, Anna and McNeil, Paul L and others, undefined
Journal: Journal of Orthopaedic Research (2017)
Neonatal CX26 removal impairs neocortical development and leads to elevated anxiety
Authors: Su, Xin and Chen, Jing-Jing and Liu, Lin-Yun and Huang, Qian and Zhang, Li-Zhao and Li, Xiao-Yang and He, Xiang-Nan and Lu, Wenlian and Sun, Shan and Li, Huawei and others, undefined
Journal: Proceedings of the National Academy of Sciences (2017): 201613237
Authors: Su, Xin and Chen, Jing-Jing and Liu, Lin-Yun and Huang, Qian and Zhang, Li-Zhao and Li, Xiao-Yang and He, Xiang-Nan and Lu, Wenlian and Sun, Shan and Li, Huawei and others, undefined
Journal: Proceedings of the National Academy of Sciences (2017): 201613237
Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging
Authors: Li, YX and Gautam, V and Brüstle, A and Cockburn, IA and Daria, VR and Gillespie, C and Gaus, K and Alt, C and Lee, WM
Journal: Journal of Biophotonics (2017)
Authors: Li, YX and Gautam, V and Brüstle, A and Cockburn, IA and Daria, VR and Gillespie, C and Gaus, K and Alt, C and Lee, WM
Journal: Journal of Biophotonics (2017)
Mechanism for Triggered Waves in Atrial Myocytes
Authors: Shiferaw, Yohannes and Aistrup, Gary L and Wasserstrom, J Andrew
Journal: Biophysical Journal (2017): 656--670
Authors: Shiferaw, Yohannes and Aistrup, Gary L and Wasserstrom, J Andrew
Journal: Biophysical Journal (2017): 656--670
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)