Name: Cal-520® NHS Ester
Brand: AAT Bioquest
SKU: 20609
Price: 465 USD
Availability: InStock

Cal-520® NHS Ester

Calcium measurement is critical for numerous biological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be the best green fluorescent calcium indicator. Conceivably this amine-reactive Cal-520® NHS ester could be coupled to an IgG or other carrier molecules to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20609	100 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	78.057 µL	390.287 µL	780.573 µL	3.903 mL	7.806 mL
5 mM	15.611 µL	78.057 µL	156.115 µL	780.573 µL	1.561 mL
10 mM	7.806 µL	39.029 µL	78.057 µL	390.287 µL	780.573 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1281.11 g/mol	=	mL	x	mM	=	mmol

Citations

View all 93 citations: Citation Explorer

Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging

Authors:

Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud

Journal:

Proceedings of the IEEE (2017): 139--157

High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging

Authors:

Daily, Neil J and Du, Zhong-Wei and Wakatsuki, Tetsuro

Journal:

ASSAY and Drug Development Technologies (2017)

HTS-Compatible Voltage-and Ca 2+-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems

Authors:

Kettenhofen, Ralf

Journal:

Stem Cell-Derived Models in Toxicology (2017): 135--152

Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes

Authors:

Servin-Vences, M Rocio and Moroni, Mirko and Lewin, Gary R and Poole, Kate

Journal:

eLife (2017): e21074

Synergistic drug combination effectively blocks Ebola virus infection

Authors:

Sun, Wei and He, Shihua and Martínez-Romero, Carles and Kouznetsova, Jennifer and Tawa, Gregory and Xu, Miao and Shinn, Paul and Fisher, Ethan G and Long, Yan and Motabar, Omid and others, undefined

Journal:

Antiviral Research (2017): 165--172

Physical properties

Molecular weight	1281.11
Solvent	DMSO

Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025