Cal-520®, potassium salt
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Dissociation constant (Kd, nM) | 320 |
Molecular weight | 921.08 |
Solvent | Water |
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Calcium Indicators, Multi-Photon Microscopy
Molecular weight 921.08 | Dissociation constant (Kd, nM) 320 | Excitation (nm) 492 | Emission (nm) 515 | Quantum yield 0.751 |
Cal-520® provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520® AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520® AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal-520™ AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520®. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520® AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520® calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists. Cal-520® sodium or potassium salt is the hydrolyzed salt of Cal-520® AM in cells. It selectively binds to calcium ion, and has the fluorescence that is strongly dependent on calcium concentration.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 108.568 µL | 542.841 µL | 1.086 mL | 5.428 mL | 10.857 mL |
5 mM | 21.714 µL | 108.568 µL | 217.136 µL | 1.086 mL | 2.171 mL |
10 mM | 10.857 µL | 54.284 µL | 108.568 µL | 542.841 µL | 1.086 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Product Family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-520®, sodium salt | 492 | 515 | 0.751 |
Cal-520FF™, potassium salt | 492 | 515 | 0.751 |
Cal-520N™, potassium salt | 492 | 515 | 0.751 |
Cal-590™, potassium salt | 574 | 588 | 0.621 |
Cal-630™, potassium salt | 609 | 626 | 0.371 |
Calbryte™ 520, potassium salt | 493 | 515 | 0.751 |
Cal-500™, potassium salt | 388 | 482 | 0.481 |
Cal-670™, potassium salt | 667 | 680 | - |
Cal-770™, potassium salt | 758 | 783 | - |
Show More (1) |
Images
Figure 1. ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-520™ AM (red curve), or Fluo-4 AM (blue curve) respectively with (left) or without probenecid (right) under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 µL per well in a Costar black wall/clear bottom 96-well plate. 100 µL of 5 µM Fluo-4 AM or Cal 520™ AM in HHBS (with or without 2.5 mm probenecid) was added into the cells, and the cells were incubated at 37 °C for 1 hour. ATP (50 μL/well) was added using FlexSation to achieve the final indicated concentrations.
Citations
View all 100 citations: Citation Explorer
Activation of the glutamatergic cingulate cortical-cortical connection facilitates pain in adult mice
Authors: Li, Xu-Hui and Shi, Wantong and Chen, Qi-Yu and Hao, Shun and Miao, Hui-Hui and Miao, Zhuang and Xu, Fang and Bi, Guo-Qiang and Zhuo, Min
Journal: Communications Biology (2023): 1247
Authors: Li, Xu-Hui and Shi, Wantong and Chen, Qi-Yu and Hao, Shun and Miao, Hui-Hui and Miao, Zhuang and Xu, Fang and Bi, Guo-Qiang and Zhuo, Min
Journal: Communications Biology (2023): 1247
L-type Ca2+ channel recovery from inactivation in rabbit atrial myocytes
Authors: Martinez-Hernandez, Elizabeth and Blatter, Lothar A and Kanaporis, Giedrius
Journal: Physiological Reports (2022): e15222
Authors: Martinez-Hernandez, Elizabeth and Blatter, Lothar A and Kanaporis, Giedrius
Journal: Physiological Reports (2022): e15222
Dendritic spikes in apical oblique dendrites of cortical layer 5 pyramidal neurons
Authors: Casta{\~n}ares, Michael Lawrence G and Stuart, Greg J and Daria, Vincent R
Journal: bioRxiv (2020)
Authors: Casta{\~n}ares, Michael Lawrence G and Stuart, Greg J and Daria, Vincent R
Journal: bioRxiv (2020)
Multiphoton minimal inertia scanning for fast acquisition of neural activity signals
Authors: Schuck, Renaud and Go, Mary Ann and Garasto, Stefania and Reynolds, Stephanie and Dragotti, Pier Luigi and Schultz, Simon R
Journal: Journal of neural engineering (2018): 025003
Authors: Schuck, Renaud and Go, Mary Ann and Garasto, Stefania and Reynolds, Stephanie and Dragotti, Pier Luigi and Schultz, Simon R
Journal: Journal of neural engineering (2018): 025003
Spatio-temporal modulation of light for stimulation and recording of neuronal activity
Authors: Ma, He and Castanares, Michael Lawrence and Daria, Vincent
Journal: (2018): 1072306
Authors: Ma, He and Castanares, Michael Lawrence and Daria, Vincent
Journal: (2018): 1072306
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators
Authors: Hashitani, Hikaru and Nguyen, Michael J and Noda, Haruka and Mitsui, Retsu and Higashi, Ryuhei and Ohta, Keisuke and Nakamura, Kei-Ichiro and Lang, Richard J
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--17
Authors: Hashitani, Hikaru and Nguyen, Michael J and Noda, Haruka and Mitsui, Retsu and Higashi, Ryuhei and Ohta, Keisuke and Nakamura, Kei-Ichiro and Lang, Richard J
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--17
Extensive Ca 2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca 2+ hypersensitivity
Authors: Uehara, Akira and Murayama, Takashi and Yasukochi, Midori and Fill, Michael and Horie, Minoru and Okamoto, Toru and Matsuura, Yoshiharu and Uehara, Kiyoko and Fujimoto, Takahiro and Sakurai, Takashi and others, undefined
Journal: The Journal of general physiology (2017): 199--218
Authors: Uehara, Akira and Murayama, Takashi and Yasukochi, Midori and Fill, Michael and Horie, Minoru and Okamoto, Toru and Matsuura, Yoshiharu and Uehara, Kiyoko and Fujimoto, Takahiro and Sakurai, Takashi and others, undefined
Journal: The Journal of general physiology (2017): 199--218
Synchronicity and Rhythmicity of Purkinje Cell Firing during Generalized Spike-and-Wave Discharges in a Natural Mouse Model of Absence Epilepsy Complex Spike Synchronicity during GSWDs
Authors: Kros, Lieke and Lindeman, S and er , undefined and Eelkman Rooda, Oscar HJ and Murugesan, Pavithra and Bina, Lorenzo and Bosman, Laurens WJ and De Zeeuw, Chris I and Hoebeek, Freek E
Journal: Frontiers in Cellular Neuroscience (2017): 346
Authors: Kros, Lieke and Lindeman, S and er , undefined and Eelkman Rooda, Oscar HJ and Murugesan, Pavithra and Bina, Lorenzo and Bosman, Laurens WJ and De Zeeuw, Chris I and Hoebeek, Freek E
Journal: Frontiers in Cellular Neuroscience (2017): 346
Simultaneous Measurement of Neural Activities of Acute Mouse Hippocampal Slices Using Multi-Electrode Array System and Laser Confocal Calcium Imaging
Authors: Hamasaki, Yuuta and Haba, Natsumi and Iwata, Naoki and Uno, Yoshiki and Saito, Minoru
Journal: Journal of Behavioral and Brain Science (2017): 68
Authors: Hamasaki, Yuuta and Haba, Natsumi and Iwata, Naoki and Uno, Yoshiki and Saito, Minoru
Journal: Journal of Behavioral and Brain Science (2017): 68
Application notes
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FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?