Name: Cal-520ER™ potassium salt
Brand: AAT Bioquest
SKU: 21132
Availability: InStock

Cal-520ER™ potassium salt

Cal-520ER™ Potassium Salt is a non-cell-permeable, low-affinity calcium indicator for monitoring Ca²⁺ changes in the endoplasmic reticulum.

ER calcium monitoring: Designed for detecting changes in Ca²⁺ levels in the endoplasmic reticulum
Low-affinity indicator: Supports measurement of fast Ca²⁺ signals and Ca²⁺ current kinetics
Non-cell-permeable format: Potassium salt form is suitable for controlled delivery or specialized intracellular calcium measurements
Research applications: Useful for studying ER calcium dynamics, intracellular calcium homeostasis, ER stress response, autophagy, and cell death-related signaling
Signal-to-noise performance: Offers improved signal-to-noise characteristics compared to Oregon Green BAPTA-5N and Fluo-4FF for ER calcium measurements

Calcium-dependent dose-response analysis of Cal-520ER™ Potassium Salt.

Datasheet

COA

Catalog	Size	Price	Quantity
21132	5x50 ug	Price
21148	10x50 ug	Price

Overview

Cal-520ER™ Potassium Salt is designed to monitor changes in calcium ion (Ca²⁺) levels in the endoplasmic reticulum (ER). The potassium salt form of Cal-520ER™ is not cell permeable and is intended for applications that require controlled intracellular delivery or specialized calcium measurement workflows.

Calcium signaling plays an important role in cell health, disease-related research, and intracellular calcium homeostasis. Ca²⁺ release from the ER and other calcium stores is involved in the activation of many cellular functions, and changes in ER Ca²⁺ content and dynamics have been associated with ER stress response, abnormal autophagy, and cell death-related pathways. Cal-520ER™ is a low-affinity Ca²⁺ indicator that can be used to record fast Ca²⁺ signals and measure the kinetics of Ca²⁺ currents. Compared to Oregon Green BAPTA-5N and Fluo-4FF, Cal-520ER™ provides improved signal-to-noise performance for ER calcium dynamics measurements.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-520ER™ potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	99.794 µL	498.972 µL	997.944 µL	4.99 mL	9.979 mL
5 mM	19.959 µL	99.794 µL	199.589 µL	997.944 µL	1.996 mL
10 mM	9.979 µL	49.897 µL	99.794 µL	498.972 µL	997.944 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1002.06 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

In Situ Visualizing Carboxylesterase Activity in Type 2 Diabetes Mellitus Using an Activatable Endoplasmic Reticulum Targetable Proximity Labeling Far-Red Fluorescent Probe.

Authors:

Xu, Ningge and Tang, Dandan and Liu, Heng and Liu, Mengyue and Wen, Zheng and Jiang, Tongmeng and Yu, Fabiao

Journal:

Analytical chemistry (2024): 10724-10731

Establishment and evaluation of targeted molecular screening model for the ryanodine receptor or sarco/endoplasmic reticulum calcium ATPase.

Authors:

Lu, Xiaopeng and Jiang, Linlin and Chen, Li and Ding, Wenwei and Wu, Hua and Ma, Zhiqing

Journal:

Pest management science (2024): 3369-3378

Imaging peroxynitrite in endoplasmic reticulum stress and acute lung injury with a near-infrared fluorescent probe.

Authors:

Yang, Ke and Liu, Ying and Deng, Min and Wang, Peipei and Cheng, Dan and Li, Songjiao and He, Longwei

Journal:

Analytica chimica acta (2024): 342050

Cypermethrin induces Sertoli cell apoptosis through endoplasmic reticulum-mitochondrial coupling involving IP3R1-GRP75-VDAC1.

Authors:

Zhang, Rui and Wang, Xu-Xu and Xie, Jia-Fei and Yao, Ting-Ting and Guo, Qian-Wen and Wang, Qi and Ding, Zhen and Zhang, Jin-Peng and Zhang, Mei-Rong and Xu, Li-Chun

Journal:

Reproductive toxicology (Elmsford, N.Y.) (2024): 108552

Use of Super-Resolution Live Cell Imaging to Distinguish Endoplasmic Reticulum: Nuclear Envelope Subcellular Localization.

Authors:

Field, Nadine and Graumann, Katja

Journal:

Methods in molecular biology (Clifton, N.J.) (2024): 285-290

Physical properties

Molecular weight	1002.06
Solvent	Water

Spectral properties

Excitation (nm)	492
Emission (nm)	515

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on August 29, 2026