Cal-590™-Dextran Conjugate *MW 10,000*
Ordering information
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Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~11000 |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 78000 |
Excitation (nm) | 563 |
Emission (nm) | 584 |
Quantum yield | 0.621 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
Cal-590™-Dextran Conjugate *MW 3,000* |
Related products
Overview | ![]() ![]() |
Molecular weight ~11000 | Extinction coefficient (cm -1 M -1) 78000 | Excitation (nm) 563 | Emission (nm) 584 | Quantum yield 0.621 |
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium. This Cal-590 dextran conjugate has Kd of 1.0 uM.
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Extinction coefficient (cm -1 M -1) | 78000 |
Excitation (nm) | 563 |
Emission (nm) | 584 |
Quantum yield | 0.621 |
Product Family
Name | Excitation (nm) | Emission (nm) |
Cal-590L® Dextran Conjugate *MW 10,000* | 569 | 590 |
Cal-630™-Dextran Conjugate *MW 3,000* | 609 | 626 |
Cal-630™-Dextran Conjugate *MW 10,000* | 609 | 626 |
Cal-520®-Dextran Conjugate *MW 3,000* | 493 | 515 |
Cal-520®-Dextran Conjugate *MW 10,000* | 493 | 515 |
Cal-670™-Dextran Conjugate *MW 3,000* | 667 | 680 |
Cal-670™-Dextran Conjugate *MW 10,000* | 667 | 680 |
Cal-770™-Dextran Conjugate *MW 3,000* | 758 | 783 |
Cal-770™-Dextran Conjugate *MW 10,000* | 758 | 783 |
Images

Figure 1. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.
Citations
View all 8 citations: Citation Explorer
Smuggling on the Nanoscale—Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo
Authors: Hoffmann, Marco and Gerlach, Sven and Takamiya, Masanari and Tarazi, Samar and Hersch, Nils and Csisz{\'a}r, Agnes and Springer, Ronald and Dreissen, Georg and Scharr, Hanno and Rastegar, Sepand and others,
Journal: Pharmaceutics (2023): 1210
Authors: Hoffmann, Marco and Gerlach, Sven and Takamiya, Masanari and Tarazi, Samar and Hersch, Nils and Csisz{\'a}r, Agnes and Springer, Ronald and Dreissen, Georg and Scharr, Hanno and Rastegar, Sepand and others,
Journal: Pharmaceutics (2023): 1210
The mammalian olfactory bulb contributes to the adaptation of odor responses: a second perceptual computation carried out by the bulb
Authors: Storace, Douglas A and Cohen, Lawrence B
Journal: Eneuro (2021)
Authors: Storace, Douglas A and Cohen, Lawrence B
Journal: Eneuro (2021)
Hypothalamic dopamine neurons control sensorimotor behavior by modulating brainstem premotor nuclei
Authors: Barrios, Joshua P and Wang, Wei-Chun and England, Roman and Reifenberg, Erica and Douglass, Adam D
Journal: bioRxiv (2020)
Authors: Barrios, Joshua P and Wang, Wei-Chun and England, Roman and Reifenberg, Erica and Douglass, Adam D
Journal: bioRxiv (2020)
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Measuring the olfactory bulb input-output transformation reveals a contribution to the perception of odorant concentration invariance
Authors: Storace, Douglas A and Cohen, Lawrence B
Journal: Nature communications (2017): 1--11
Authors: Storace, Douglas A and Cohen, Lawrence B
Journal: Nature communications (2017): 1--11
α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)
Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?