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Cal-590™-Dextran Conjugate *MW 10,000*

The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.
The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium.
Ordering information
Price ()
Catalog Number20509
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight~11000
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)78000
Excitation (nm)563
Emission (nm)584
Quantum yield0.621
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Show More (76)

OverviewpdfSDSpdfProtocol


Molecular weight
~11000
Extinction coefficient (cm -1 M -1)
78000
Excitation (nm)
563
Emission (nm)
584
Quantum yield
0.621
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590 dextran conjugates might be a better choice than other red fluorescent dextrtan conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium. This Cal-590 dextran conjugate has Kd of 1.0 uM.

Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)78000
Excitation (nm)563
Emission (nm)584
Quantum yield0.621

Citations


View all 7 citations: Citation Explorer
Hypothalamic dopamine neurons control sensorimotor behavior by modulating brainstem premotor nuclei
Authors: Barrios, Joshua P and Wang, Wei-Chun and England, Roman and Reifenberg, Erica and Douglass, Adam D
Journal: bioRxiv (2020)
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Thillaiappan, Nagendra Babu and Chavda, Alap P and Tovey, Stephen C and Prole, David L and Taylor, Colin W
Journal: Nature Communications (2017): 1505
Measuring the olfactory bulb input-output transformation reveals a contribution to the perception of odorant concentration invariance
Authors: Storace, Douglas A and Cohen, Lawrence B
Journal: Nature communications (2017): 1--11
α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
In vivo deep two-photon imaging of neural circuits with the fluorescent Ca2+ indicator Cal-590
Authors: Tischbirek, Carsten H and Birkner, Antje and Konnerth, Arthur
Journal: The Journal of Physiology (2016)
Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator
Authors: Tischbirek, Carsten and Birkner, Antje and Jia, Hongbo and Sakmann, Bert and Konnerth, Arthur
Journal: Proceedings of the National Academy of Sciences (2015): 11377--11382