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Cal-590L® Dextran Conjugate *MW 10,000*

Among the fluorescent calcium indicator dextran conjugates, Cal-590L dextran conjugates might be a better choice than other red fluorescent dextran conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium. Cal-590L dextran has Kd ~230 uM.
Among the fluorescent calcium indicator dextran conjugates, Cal-590L dextran conjugates might be a better choice than other red fluorescent dextran conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium. Cal-590L dextran has Kd ~230 uM.
Ordering information
Price ()
Catalog Number20525
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Spectral properties
Excitation (nm)569
Emission (nm)590
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Excitation (nm)
569
Emission (nm)
590
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant salt forms of these dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-590L dextran conjugates might be a better choice than other red fluorescent dextran conjugates due to its higher fluorescence quantum yield and larger fluorescence enhancement by calcium. Cal-590L dextran has Kd ~230 uM.

Spectrum


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spectrum

Spectral properties

Excitation (nm)569
Emission (nm)590

References


View all 9 references: Citation Explorer
In vivo nuclear Ca2+-ATPase phosphorylation triggers intermediate size molecular transport to the nucleus.
Authors: Gensburger, Claire and Freyermuth, Solange and Klein, Christian and Malviya, Anant N
Journal: Biochemical and biophysical research communications (2003): 1225-8
Real time, confocal imaging of Ca(2+) waves in arterially perfused rat hearts.
Authors: Baader, Andreas P and Büchler, Lorenz and Bircher-Lehmann, Lilly and Kléber, André G
Journal: Cardiovascular research (2002): 105-15
Assessment and validation of a microinjection method for kinetic analysis of [Ca2+]i in individual cells undergoing apoptosis.
Authors: Tombal, B and Denmeade, S R and Isaacs, J T
Journal: Cell calcium (1999): 19-28
Effects of adenosine on Ca2+ entry in the nerve terminal of the frog neuromuscular junction.
Authors: Robitaille, R and Thomas, S and Charlton, M P
Journal: Canadian journal of physiology and pharmacology (1999): 707-14
The nucleus of HeLa cell contains tubular structures for Ca2+ signalling.
Authors: Lui, P P and Kong, S K and Kwok, T T and Lee, C Y
Journal: Biochemical and biophysical research communications (1998): 88-93
The rise of nuclear and cytosolic Ca2+ can be uncoupled in HeLa cells.
Authors: Lui, P P and Kong, S K and Fung, K P and Lee, C Y
Journal: Pflugers Archiv : European journal of physiology (1998): 371-6
Porcine aortic endothelial gap junctions: identification and permeation by caged InsP3.
Authors: Carter, T D and Chen, X Y and Carlile, G and Kalapothakis, E and Ogden, D and Evans, W H
Journal: Journal of cell science (1996): 1765-73
Calcium signals in and around the nucleus in sea urchin eggs.
Authors: Gillot, I and Whitaker, M
Journal: Cell calcium (1994): 269-78
Spontaneous and propagated calcium release in isolated cardiac myocytes viewed by confocal microscopy.
Authors: Williams, D A and Delbridge, L M and Cody, S H and Harris, P J and Morgan, T O
Journal: The American journal of physiology (1992): C731-42