Cal-670™, potassium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cal-670™ is a near infrared (NIR) calcium indicator with maximum emission at ~675 nm. It can be well excited with the red lasers at 633 nm or 647 nm with a moderate calcium affinity of Kd ~853 nM. Cal-670™ is one of the very few calcium indicators that can be potentially used for in vivo imaging since it has a NIR fluorescence.

Cal-670 was incubated with buffer that contains different concentration of free Ca2+. The fluorescence was monitored on fluorimeter GeminiXS (Molecular Device) at 650 nm/ 675 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20455	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cal-670™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	62.973 µL	314.863 µL	629.727 µL	3.149 mL	6.297 mL
5 mM	12.595 µL	62.973 µL	125.945 µL	629.727 µL	1.259 mL
10 mM	6.297 µL	31.486 µL	62.973 µL	314.863 µL	629.727 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1587.99 g/mol	=	mL	x	mM	=	mmol

Citations

View all 12 citations: Citation Explorer

All-optical crosstalk-free manipulation and readout of Chronos-expressing neurons

Authors:

Soor, Navjeevan S and Quicke, Peter and Howe, Carmel L and Pang, Kuin T and Neil, Mark AA and Schultz, Simon R and Foust, Amanda J

Journal:

Journal of physics D: Applied physics (2019): 104002

All-Optical Crosstalk-Free Manipulation and Readout of Chronos-expressing Neurons

Authors:

Soor, Navjeevan Singhh and Quicke, Peter and Howe, Carmel L and Pang, Kuin Tian and Neil, Mark and Schultz, Simon and Foust, Am and a Joy, undefined

Journal:

Journal of Physics D: Applied Physics (2018)

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells

Authors:

Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined

Journal:

Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

Authors:

Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang

Journal:

Scientific Reports (2017): 41781

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors:

Zhang Z, Kitching P.

Journal:

J Virol Methods (2000): 187

Physical properties

Dissociation constant (K_d, nM)	853
Molecular weight	1587.99
Solvent	Water

Spectral properties

Excitation (nm)	667
Emission (nm)	680

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025