Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Cal Red™ R525/650 potassium salt

Graph illustrates signal-to-noise ratio (SNR) x 100%. ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells incubated with Cal Red R525/650. ATP (50 uL/well) was added by FlexStation3 (Molecular Devices) to achieve the final indicated concentrations.
Graph illustrates signal-to-noise ratio (SNR) x 100%. ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells incubated with Cal Red R525/650. ATP (50 uL/well) was added by FlexStation3 (Molecular Devices) to achieve the final indicated concentrations.
Ordering information
Price ()
Catalog Number20588
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Dissociation constant (Kd, nM)330
Molecular weight~1000
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Related products
Cal Red™ R525/650 AM
Chemical Phosphorylation Reagent I (CPR I)
Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*
Cal Green™ 1, hexapotassium salt
Cal Green™ 1, AM [Equivalent to Calcium Green-1, AM]
Cal-590™-Dextran Conjugate *MW 3,000*
Cal-590™-Dextran Conjugate *MW 10,000*
Cal-590™ AM
Cal-590™, sodium salt
Cal-590™, potassium salt
Cal-630™ AM
Cal-630™, sodium salt
Cal-630™, potassium salt
Cal-630™-Dextran Conjugate *MW 3,000*
Cal-630™-Dextran Conjugate *MW 10,000*
Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*
Fluo-4, Pentapotassium Salt
Cal-520®-Dextran Conjugate *MW 3,000*
Cal-520®-Dextran Conjugate *MW 10,000*
Cal-520®-Biotin Conjugate
Cal-520®-Biocytin Conjugate
Cal-520® NHS Ester
Cal-520® maleimide
Fluo-3, AM *CAS 121714-22-5*
Fluo-3, AM *UltraPure grade* *CAS 121714-22-5*
Fluo-3, AM *Bulk package* *CAS 121714-22-5*
Fluo-3FF, AM *UltraPure grade* *Cell permeant*
Fluo-3, pentasodium salt
Fluo-3, pentapotassium salt
Fluo-3, pentaammonium salt
Fluo-3FF, pentapotassium salt
Fluo-8®, AM
Fluo-8®, sodium salt
Fluo-8®, potassium salt
Fluo-8H™, AM
Fluo-8H™, sodium salt
Fluo-8L™, AM
Fluo-8L™, sodium salt
Fluo-8L™, potassium salt
Fluo-8FF™, potassium salt
Fluo-8FF™, AM
Cal-520®, AM
Cal-520®, sodium salt
Cal-520®, potassium salt
Cal-520FF™, AM
Cal-520FF™, potassium salt
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines*
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit
Mag-Fluo-4 potassium salt
Mag-Fluo-4 AM
Fluo-2, potassium salt
Fluo-2, AM
Fluo-5F, AM *Cell permeant*
Fluo-5F, pentapotassium Salt *Cell impermeant*
Fluo-5N, AM *Cell permeant*
Fluo-5N, pentapotassium Salt *Cell impermeant*
Cal-520N™, AM
Cal-520N™, potassium salt
Screen Quest™ Fluo-4 No Wash Calcium Assay Kit
Calbryte™ 520 AM
Calbryte™ 520, potassium salt
Calbryte™ 590 AM
Calbryte™ 590, potassium salt
Calbryte™ 630 AM
Calbryte™ 630, potassium salt
Screen Quest™ Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit
Screen Quest™ Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit
Calbryte™-520L AM
Calbryte™-520L, potassium salt
Cal-500™, potassium salt
Cal-500™ AM
Cal-670™, potassium salt
Cal-670™-Dextran Conjugate *MW 3,000*
Cal-670™-Dextran Conjugate *MW 10,000*
Cal-770™, potassium salt
Cal-770™-Dextran Conjugate *MW 3,000*
Cal-770™-Dextran Conjugate *MW 10,000*
Calbryte™-520XL azide
Calbryte™-520XL, potassium salt
Calbryte™-520XL AM
Calbryte™-520XL-Dextran
Cal-520L®-Dextran Conjugate *MW 10,000*
RatioWorks™ Cal-520L®/Cy5-Dextran Conjugate *MW 10,000*
Cal-520L™ maleimide
RatioWorks™ Cal-520®/zFluor 647™ -Dextran Conjugate *MW 10,000*
Cal-590L® Dextran Conjugate *MW 10,000*
RatioWorks™ Cal-590L®/Cy5-Dextran Conjugate *MW 10,000*
Show More (87)

OverviewpdfSDSpdfProtocol


Molecular weight
~1000
Dissociation constant (Kd, nM)
330
The intracellular calcium flux assay is a widely used method for monitoring the activities of GPCRs and calcium channels. To quantify the intracellular calcium concentration, ratiometric fluorescent calcium indicators are preferred because the ratio is directly related to the calcium concentration and independent of the cell numbers and dye loading concentration. However, the most popular ratiometric calcium indicators (such as Fura-2 and Indo-1) have certain limitations such as lower sensitivity, UV excitation, and not compatible with HTS screening filter set. Cal Red™ R525/650 has been developed as a new 488 nm-excitable ratiometric fluorescence calcium indicator. Cal Red™ R525/650 is well excited at 488 nm with two emissions at 525 nm and 650 nm. Upon binding calcium, the emission signal of Cal Red™ R525/650 is increased at 525 nm and decreased at 650 nm when excited at 488 nm. The excitation and emission wavelength of Cal Red™ R525/650 are compatible with common filter sets with minimal damage to cells, making it a robust tool for evaluating and screening GPCR agonists and antagonists as well as calcium channel targets.

References


View all 12 references: Citation Explorer
Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets
Authors: Wendt ER, Ferry H, Greaves DR, Keshav S.
Journal: PLoS One (2015): e0119532
A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23
Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red
Authors: Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.
Journal: Shi Yan Sheng Wu Xue Bao (1998): 323
Problems associated with using Fura-2 to measure free intracellular calcium concentrations in human red blood cells
Authors: Blackwood AM, Sagnella GA, Mark and u ND, MacGregor GA.
Journal: J Hum Hypertens (1997): 601
Calcium transient alternans in blood-perfused ischemic hearts: observations with fluorescent indicator fura red
Authors: Wu Y, Clusin WT.
Journal: Am J Physiol (1997): H2161
IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes
Authors: Floto RA, Mahaut-Smith MP, Somasundaram B, Allen JM.
Journal: Cell Calcium (1995): 377
Localization of calcium entry through calcium channels in olfactory receptor neurones using a laser scanning microscope and the calcium indicator dyes Fluo-3 and Fura-Red
Authors: Schild D, Jung A, Schultens HA.
Journal: Cell Calcium (1994): 341
Improved sensitivity in flow cytometric intracellular ionized calcium measurement using fluo-3/Fura Red fluorescence ratios
Authors: Novak EJ, Rabinovitch PS.
Journal: Cytometry (1994): 135
The distribution of intracellular calcium chelator (fura-2) in a population of intact human red cells
Authors: Lew VL, Etzion Z, Bookchin RM, daCosta R, Vaananen H, Sassaroli M, Eisinger J.
Journal: Biochim Biophys Acta (1993): 152