Name: Calbryte™ 520, potassium salt
Brand: AAT Bioquest
SKU: 20656
Availability: InStock

Calbryte™ 520, potassium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in cellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Followed by Fluo-3 being introduced in 1989, Fluo-4, Fluo-8 and Cal-520 were later developed with improved signal/background ratio. However, there are still a few severe problems with Fluo-4. For example, as for Fluo-3, in all most all the intracellular calcium assays with Fluo-4, probenecid is required to prevent the cell-loaded Fluo-4 from leaking out of cells. The use of probenecid with Fluo-4-based calcium assays compromise the assay results since probenecid is well-documented to have a variety of complicated cellular effects. Calbryte™ 520 is a new generation of fluorescent indicator for calcium measurements, its greatly improved signal-to-noise ratio and better intracellular retention properties make Calbryte™ 520 the most robust calcium indicator. It has the identical excitation and emission wavelength as Fluo-4, thus the same Fluo-4 assay settings can be readily applied to Calbryte™ 520-based calcium assays.

<p>Fluorescence emission spectra of Calbryte™ 520 in solution containing 0 to 39 µM free Ca<sup>2+</sup>.</p>

Protocol

COA

Catalog	Size	Price
20656	2x50 ug	Price
20657	1 mg	Price
20658	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Calbryte™ 520, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	110.009 µL	550.043 µL	1.1 mL	5.5 mL	11.001 mL
5 mM	22.002 µL	110.009 µL	220.017 µL	1.1 mL	2.2 mL
10 mM	11.001 µL	55.004 µL	110.009 µL	550.043 µL	1.1 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	909.02 g/mol	=	mL	x	mM	=	mmol

Citations

View all 12 citations: Citation Explorer

Ageing-induced weakness of mouse NMJs is associated with reduced active zone density, synaptic event kinetics and presynaptic calcium entry

Authors:

Li, Yizhi and Case, Elinor H and Blanchard, Christopher and Monteleone, Anna and Gandhi, Meera and Jaie, Anousha and Badawi, Yomna and Meriney, Stephen D

Journal:

The Journal of Physiology (2025)

Calcium signals in the murine zona glomerulosa

Authors:

Dinh, Hoang An

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells

Authors:

Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined

Journal:

Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

Authors:

Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang

Journal:

Scientific Reports (2017): 41781

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors:

Zhang Z, Kitching P.

Journal:

J Virol Methods (2000): 187

Physical properties

Dissociation constant (K_d, nM)	1200
Molecular weight	909.02
Solvent	Water

Spectral properties

Excitation (nm)	493
Emission (nm)	515
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 15, 2025