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Calbryte™-520L, potassium salt
The intracellular calcium flux assay is a widely used method for monitoring signal transduction pathways and high throughput screening of G protein-coupled receptors (GPCRs) and calcium channel targets. Following the introduction of Fluo-3 in 1989, Fluo-4, Fluo-8, and Cal-520 were later developed with enhanced signal-to-background ratios and soon became the Ca2+ indicators of choice for confocal microscopy, flow cytometry, and high throughput screening applications. Calbryte 520L has a low affinity to calcium ions with a Kd ≤91 µM. Calbryte 520L produces a bright fluorescence signal in the presence of calcium at a high concentration. It has an identical excitation and emission wavelength as Fluo-4; thus, the same Fluo-4 assay settings can be readily applied to Calbryte 520L-based calcium assays.
Ca2+ Dependent Dose Response of Calbryte 520
Ca2+ Dependent Dose Response of Calbryte 520
CatalogSize
Price
Quantity
2064210X50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)91000
Molecular weight794.88
SolventWater
Spectral properties

Excitation (nm)493
Emission (nm)515
Quantum yield
0.75
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 13, 2025