Calbryte™-520XL azide

Calbryte 520XL is a new fluorescent calcium indicator with extremely low affinity (Kd ~300 uM) which is the similar to the well-known Rhod 5N, but much more stable than Rhod-5N. Calbryte 520XL produces bright fluorescence signal in the presence of calcium ion in high concentration. It has the identical excitation and emission wavelength as Fluo-4, thus the same Fluo-4 assay settings can be readily applied to Calbryte 520XL-based calcium assays. Calbryte 520XL is an excellent alternative to Rhod-5N. Calbryte 520XL azide can be readily conjugated to a carrier through the well-known click chemistry.

Ca2+ dependent fluorescence emission of Calbryte™-520XL indicator (Ex/Em = 490/525 nm).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20643	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Calbryte™-520XL azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.546 µL	442.729 µL	885.457 µL	4.427 mL	8.855 mL
5 mM	17.709 µL	88.546 µL	177.091 µL	885.457 µL	1.771 mL
10 mM	8.855 µL	44.273 µL	88.546 µL	442.729 µL	885.457 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1129.36 g/mol	=	mL	x	mM	=	mmol

Citations

View all 13 citations: Citation Explorer

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells

Authors:

Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined

Journal:

Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

Authors:

Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang

Journal:

Scientific Reports (2017): 41781

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons

Authors:

Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei

Journal:

Int J Clin Exp Med (2017): 6861--6868

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection

Authors:

Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun

Journal:

Journal of cellular and molecular medicine (2015): 581--594

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors:

Zhang Z, Kitching P.

Journal:

J Virol Methods (2000): 187

Physical properties

Dissociation constant (K_d, nM)	300000
Molecular weight	1129.36
Solvent	DMSO

Spectral properties

Excitation (nm)	493
Emission (nm)	515
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025