Name: Calbryte™ 590, potassium salt
Brand: AAT Bioquest
SKU: 20706
Availability: InStock

Calbryte™ 590, potassium salt

The intracellular calcium flux assay is a widely used method in monitoring signal transduction pathways and high throughput screening of G protein“coupled receptors (GPCRs) and calcium channel targets. Followed by Rhod-2 being introduced in 1989, Rhod-4 and Cal-590 were later developed with improved signal/background ratio, and they became the widely used red fluorescent Ca2+ indicators for confocal microscopy, flow cytometry and high throughput screening applications. Calbryte™ 590 is a new generation of red fluorescent indicators for the measurement of intracellular calcium. Its greatly improved signal/background ratio and intracellular retention properties make Calbryte™ 590 the most robust red fluorescent indicator for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists in live cells.

<p>Fluorescence emission spectra of Calbryte™ 590 in solution containing 0 to 39 µM free Ca<sup>2+</sup>.</p>

Datasheet

Catalog	Size	Price	Quantity
20706	5x50 ug	Price
20707	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Calbryte™ 590, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	93.025 µL	465.125 µL	930.25 µL	4.651 mL	9.302 mL
5 mM	18.605 µL	93.025 µL	186.05 µL	930.25 µL	1.86 mL
10 mM	9.302 µL	46.512 µL	93.025 µL	465.125 µL	930.25 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1074.98 g/mol	=	mL	x	mM	=	mmol

Citations

View all 14 citations: Citation Explorer

Perilymphatic ATP plays a critical role in modulating cochlear function to protect from loud sound induced hearing loss

Authors:

Prasad, Sonal and Karlsson, Urban and Pitk{\"a}nen, Marja and Fridberger, Anders

Journal:

eBioMedicine (2026)

The tectorial membrane has a critical role in metabolic age-related hearing loss

Authors:

Prasad, Sonal and Pitk{\"a}nen, Marja and Fridberger, Anders

Journal:

EBioMedicine (2025)

Divergent actions of CaV1. 1 voltage-sensing domain III in activating EC coupling and channel gating

Authors:

Pelizzari, Simone and Campiglio, Marta and Fern{\'a}ndez-Quintero, Monica L and Heiss, Martin C and Tuluc, Petronel and Flucher, Bernhard E

Journal:

Biophysical Journal (2025)

CaV1. 1 voltage-sensing domain III exclusively controls skeletal muscle excitation-contraction coupling

Authors:

Pelizzari, Simone and Heiss, Martin C and Fern{\'a}ndez-Quintero, Monica L and El Ghaleb, Yousra and Liedl, Klaus R and Tuluc, Petronel and Campiglio, Marta and Flucher, Bernhard E

Journal:

Nature Communications (2024): 7440

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors:

Zhang Z, Kitching P.

Journal:

J Virol Methods (2000): 187

Physical properties

Dissociation constant (K_d, nM)	1400
Molecular weight	1074.98
Solvent	Water

Spectral properties

Excitation (nm)	581
Emission (nm)	593

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on July 17, 2026