Name: Calbryte™ 590, potassium salt
Brand: AAT Bioquest
SKU: 20706
Availability: InStock

Calbryte™ 590, potassium salt

The intracellular calcium flux assay is a widely used method in monitoring signal transduction pathways and high throughput screening of G protein“coupled receptors (GPCRs) and calcium channel targets. Followed by Rhod-2 being introduced in 1989, Rhod-4 and Cal-590 were later developed with improved signal/background ratio, and they became the widely used red fluorescent Ca2+ indicators for confocal microscopy, flow cytometry and high throughput screening applications. Calbryte™ 590 is a new generation of red fluorescent indicators for the measurement of intracellular calcium. Its greatly improved signal/background ratio and intracellular retention properties make Calbryte™ 590 the most robust red fluorescent indicator for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists in live cells.

<p>Fluorescence emission spectra of Calbryte™ 590 in solution containing 0 to 39 µM free Ca<sup>2+</sup>.</p>

Protocol

COA

Catalog	Size	Price	Quantity
20706	5x50 ug	Price
20707	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Calbryte™ 590, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	93.025 µL	465.125 µL	930.25 µL	4.651 mL	9.302 mL
5 mM	18.605 µL	93.025 µL	186.05 µL	930.25 µL	1.86 mL
10 mM	9.302 µL	46.512 µL	93.025 µL	465.125 µL	930.25 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1074.98 g/mol	=	mL	x	mM	=	mmol

Citations

View all 11 citations: Citation Explorer

CaV1. 1 voltage-sensing domain III exclusively controls skeletal muscle excitation-contraction coupling

Authors:

Pelizzari, Simone and Heiss, Martin C and Fern{\'a}ndez-Quintero, Monica L and El Ghaleb, Yousra and Liedl, Klaus R and Tuluc, Petronel and Campiglio, Marta and Flucher, Bernhard E

Journal:

Nature Communications (2024): 7440

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells

Authors:

Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined

Journal:

Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

Authors:

Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang

Journal:

Scientific Reports (2017): 41781

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons

Authors:

Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei

Journal:

Int J Clin Exp Med (2017): 6861--6868

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors:

Zhang Z, Kitching P.

Journal:

J Virol Methods (2000): 187

Physical properties

Dissociation constant (K_d, nM)	1400
Molecular weight	1074.98
Solvent	Water

Spectral properties

Excitation (nm)	581
Emission (nm)	593

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025