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Calbryte™ 630, potassium salt
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. x-Rhod-1 is commonly used as a red fluorescent calcium indicator. However, x-Rhod-1 is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Calbryte™ 630 has been developed to improve x-Rhod-1 cell loading and calcium response while maintaining the spectral wavelength of x-Rhod-1, making it compatible with Texas Red® filter set. In CHO and HEK cells Calbryte™ 630 AM has cellular calcium response that is much more sensitive than x-Rhod-1. The spectra of Calbryte™ 630 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.
<p>Fluorescence emission spectra of Calbryte&trade; 630 in solution containing 0 to 39 &micro;M free Ca<sup>2+</sup>.</p>
<p>Fluorescence emission spectra of Calbryte&trade; 630 in solution containing 0 to 39 &micro;M free Ca<sup>2+</sup>.</p>
CatalogSize
Price
Quantity
207275x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)1200
Molecular weight1091.06
SolventWater
Spectral properties

Excitation (nm)607
Emission (nm)624
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 13, 2025