Calcein AM | AAT Bioquest

Calcein Blue, AM

CAS 168482-84-6

It is a cell-permeable version of calcein blue. Upon getting into live cells the weakly fluorescent calcein blue AM is hydrolyzed into calcein blue that has the excitation/emission maxima similar to those of DAPI, Hoechst and AMCA. This exceptional spectral separation from the typical green and red fluorophores (such as FITC, TMR and Texas Red) provides additional options for multiplexing experiments. Because calcein blue AM is intrinsically fluorescent, a proper filter set and additional wash step may be necessary to minimize background fluorescence. Our calcium assay buffers may be used to effectively wash extracellular calcein blue.

Protocol

SDS

Catalog	Size	Price	Quantity
22007	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Calcein Blue, AM *CAS 168482-84-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	214.864 µL	1.074 mL	2.149 mL	10.743 mL	21.486 mL
5 mM	42.973 µL	214.864 µL	429.729 µL	2.149 mL	4.297 mL
10 mM	21.486 µL	107.432 µL	214.864 µL	1.074 mL	2.149 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	465.41 g/mol	=	mL	x	mM	=	mmol

Citations

View all 19 citations: Citation Explorer

Titrating chimeric antigen receptors on CAR T cells enabled by a microfluidic-based dosage-controlled intracellular mRNA delivery platform

Authors:

Chen, Yu-Hsi and Mirza, Mahnoor and Jiang, Ruoyu and Lee, Abraham P

Journal:

Biomicrofluidics (2024)

3D Bioprinting of Prevascularized Full-Thickness Gelatin-Alginate Structures with Embedded Co-Cultures

Authors:

B{\"o}ttcher, Bastian and Pflieger, Astrid and Schumacher, Jan and Jungnickel, Berit and Feller, Karl-Heinz

Journal:

Bioengineering (2022): 242

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice

Authors:

Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang

Journal:

Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation

Authors:

Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined

Journal:

Cellular Signalling (2017)

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B

Authors:

Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres

Journal:

Scientific reports (2016)

References

View all 84 references: Citation Explorer

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Authors:

Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang

Journal:

Biochemical and Biophysical Research Communications (2017)

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine

Authors:

Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.

Journal:

Biophys J. (2006)

A vaccination and challenge model using calcein marked fish

Authors:

Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.

Journal:

Fish Shellfish Immunol (2006): 20

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging

Authors:

Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.

Journal:

Cytometry A (2005): 78

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake

Authors:

Schoonen WG, Westerink WM, de Roos JA, Debiton E.

Journal:

Toxicol In Vitro (2005): 505

Physical properties

Molecular weight	465.41
Solvent	DMSO

Spectral properties

Excitation (nm)	354
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	168482-84-6

Instrument settings

Flow cytometer
Excitation	350 nm or 405 nm laser
Emission	450/40 nm filter
Instrument specification(s)	Pacific Blue channel

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	360
Emission	450
Cutoff	420
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Cell Viability & Proliferation
Flyer: Calcein Dyes

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Page updated on January 23, 2025