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Calcein Deep Red™ acetate
This product is now replaced by #22011. Calcein AM is one the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications. For example, it is impossible to use Calcein AM in combination of GFP-tranfacted cells due to the same color to GFP. To address this color limitation of Calcein AM, we have developed Calcein Orange™, Calcein Red™ and Calcein Deep Red™. These new Calcein AM analogs enable the multicolor labeling and functional analysis of live cells in combination with Calcein AM. Non-fluorescent Calcein Deep Red™ acetate can easily get into live cells and hydrolyzes to generate strongly fluorescent Calcein Deep Red™ (Cat#: 21902) dye. Calcein Deep Red™ dye can be monitored with the common Cy5 filter set.
Images of Live HeLa cells stained with Calcein Deep Red™ acetate (Cat.22010 ). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).
Images of Live HeLa cells stained with Calcein Deep Red™ acetate (Cat.22010 ). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).
CatalogSize
Price
Quantity
220101 mg
Price
 
Physical properties

Molecular weight530.36
SolventDMSO
Spectral properties

Excitation (nm)643
Emission (nm)663
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel

Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation620
Emission660
Cutoff630
Recommended plateSolid black
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Page updated on January 23, 2025