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Calcein Orange™ diacetate
Calcein AM is one of the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications. For example, it is impossible to use Calcein AM in combination of GFP-tranfacted cells due to the same color to GFP. To address this color limitation of Calcein AM, we have developed Calcein Orange™, Calcein Red™ and Calcein Red™. These new Calcein AM analogs enable the multicolor labeling and functional analysis of live cells in combination with Calcein AM. Non-fluorescent Calcein Orange™ diaceate can easily get into live cells and hydrolyzes to generate strongly fluorescent Calcein Orange™ dye. AAT Bioquest also offers Calcein Orange™ as a reference dye to Calcein Orange™ diacetate.
Image of Live HeLa cells stained with&nbsp;Calcein Orange&trade; diacetate (Cat#22009). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).<br />&nbsp;<br />&nbsp;
Image of Live HeLa cells stained with&nbsp;Calcein Orange&trade; diacetate (Cat#22009). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).<br />&nbsp;<br />&nbsp;
CatalogSize
Price
Quantity
220091 mg
Price
 
Physical properties

Molecular weight913.266
SolventDMSO
Spectral properties

Excitation (nm)531
Emission (nm)545
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation488/532 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel

Fluorescence microscope
ExcitationTRITC filter set
EmissionTRITC filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation520
Emission550
Cutoff530
Recommended plateSolid black
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Page updated on January 23, 2025