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Calcein Red™ AM
Calcein AM is one of the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications. For example, it is impossible to use Calcein AM in combination of GFP-tranfacted cells due to the same color to GFP. To address this color limitation of Calcein AM, we have developed Calcein Orange™, Calcein Red™ and Calcein Deep Red™. These new Calcein AM analogs enable the multicolor labeling and functional analysis of live cells in combination with Calcein AM. Non-fluorescent Calcein Red™ AM can easily get into live cells and hydrolyzes to generate strongly fluorescent Calcein Red™ dye. Calcein Red™ dye can be monitored with the common TRITC/Cy3 filter set. AAT Bioquest offers Calcein Red™ as a reference dye to Calcein Red™ AM.
Images of Live HeLa cells stained with Calcein Red <sup>TM</sup>, AM (Cat.21900). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).
Images of Live HeLa cells stained with Calcein Red <sup>TM</sup>, AM (Cat.21900). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17535).
CatalogSize
Price
Quantity
219001 mg
Price
 
Physical properties

Molecular weight1015.79
SolventDMSO
Spectral properties

Excitation (nm)562
Emission (nm)576
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation532/561 nm laser
Emission585/40 nm filter

Fluorescence microscope
ExcitationTRITC filter set
EmissionTRITC filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation540
Emission590
Cutoff570
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on January 23, 2025