Calcein | AAT Bioquest

Calcein Red™ sodium salt

Calcein AM is one of the most popular fluorescent probes used for labeling and monitoring cellular functions of live cells. However, the single color of Calcein AM makes it impossible to use this valuable reagent in the multicolor applications. For example, it is impossible to use Calcein AM in combination of GFP-tranfacted cells due to the same color to GFP. To address this color limitation of Calcein AM, we have developed Calcein Orange™, Calcein Red™ and Calcein Red™. These new Calcein AM analogs enable the multicolor labeling and functional analysis of live cells in combination with Calcein AM. Non-fluorescent Calcein Red™ AM can easily get into live cells and hydrolyzes to generate strongly fluorescent Calcein Red™ dye. Calcein Red™ dye can be monitored with the common TRITC/Cy3 filter set. AAT Bioquest also offers Calcein Red™ as a reference dye to Calcein Red™ AM.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21901	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Calcein Red™ sodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.148 µL	605.738 µL	1.211 mL	6.057 mL	12.115 mL
5 mM	24.23 µL	121.148 µL	242.295 µL	1.211 mL	2.423 mL
10 mM	12.115 µL	60.574 µL	121.148 µL	605.738 µL	1.211 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	825.44 g/mol	=	mL	x	mM	=	mmol

Citations

View all 17 citations: Citation Explorer

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice

Authors:

Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang

Journal:

Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation

Authors:

Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined

Journal:

Cellular Signalling (2017)

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B

Authors:

Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres

Journal:

Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water

Authors:

Alharbi, Hattan A and Saunders, David MV and Al-Mousa, Ahmed and Alcorn, Jane and Pereira, Alberto S and Martin, Jonathan W and Giesy, John P and Wiseman, Steve B

Journal:

Aquatic Toxicology (2016): 81--88

Rapid generation of collagen-based microtissues to study cell--matrix interactions

Authors:

Brett, Marie-Elena and Crampton, Alex and ra L , undefined and Wood, David K

Journal:

Technology (2016): 1--8

References

View all 84 references: Citation Explorer

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Authors:

Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang

Journal:

Biochemical and Biophysical Research Communications (2017)

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine

Authors:

Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.

Journal:

Biophys J. (2006)

A vaccination and challenge model using calcein marked fish

Authors:

Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.

Journal:

Fish Shellfish Immunol (2006): 20

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging

Authors:

Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.

Journal:

Cytometry A (2005): 78

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake

Authors:

Schoonen WG, Westerink WM, de Roos JA, Debiton E.

Journal:

Toxicol In Vitro (2005): 505

Physical properties

Molecular weight	825.44
Solvent	Water

Spectral properties

Excitation (nm)	562
Emission (nm)	576

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on January 23, 2025