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Calcein UltraBlue™ AM

Fluorescence images of HeLa cells stained with Calcein UltraBlue™ AM (upper row) or Calcein Blue AM (lower row) in a Costar black wall/clear bottom 96-well plate. Left: Live HeLa cells in HH buffer; Middle: Live HeLa cells in medium; Right: Fixed HeLa cells.
Fluorescence images of HeLa cells stained with Calcein UltraBlue™ AM (upper row) or Calcein Blue AM (lower row) in a Costar black wall/clear bottom 96-well plate. Left: Live HeLa cells in HH buffer; Middle: Live HeLa cells in medium; Right: Fixed HeLa cells.
Fluorescence images of HeLa cells stained with Calcein UltraBlue™ AM (upper row) or Calcein Blue AM (lower row) in a Costar black wall/clear bottom 96-well plate. Left: Live HeLa cells in HH buffer; Middle: Live HeLa cells in medium; Right: Fixed HeLa cells.
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Physical properties
Molecular weight802.68
SolventDMSO
Spectral properties
Excitation (nm)359
Emission (nm)458
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
802.68
Excitation (nm)
359
Emission (nm)
458
Calcein UltraBlue™ AM is a cell-permeable version of Calcein UltraBlue™. Upon getting into live cells the weakly fluorescent Calcein UltraBlue™ AM is hydrolyzed into Calcein UltraBlue™ that has the excitation/emission maxima similar to those of Calcein Blue, DAPI, Hoechst and AMCA. This exceptional spectral separation from the typical green and red fluorophores (such as FITC, TMR and Texas Red) provides additional options for multiplexing experiments. Calcein UltraBlue™ has similar spectral properties to those of Calcein Blue. However, compared with Calcein Blue, Calcein UltraBlue™ has higher photostability and stronger fluorescence intensity at physiological pH, making it a more robust fluorescent probe than Calcein Blue.

Platform


Flow cytometer

Excitation350/405 nm laser
Emission450/40 nm filter
Instrument specification(s)Pacific Blue channel

Fluorescence microscope

ExcitationDAPI filter set
EmissionDAPI filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader

Excitation360
Emission450
Cutoff420
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode

Example protocol


PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Calcein UltraBlue™ AM Stock Solution
Prepare a 2 to 5 mM stock solution of Calcein UltraBlue™ AM in high-quality, anhydrous DMSO.
Note     The nonionic detergent Pluronic® F-127 can be used to increase the aqueous solubility of AM esters. In the staining buffer, the final Pluronic® F-127 concentration should be approximately 0.02%. A variety of Pluronic® F-127 products can be purchased from AAT Bioquest. Avoid long-term storage of AM esters in the presence of Pluronic® F-127.

PREPARATION OF WORKING SOLUTION

Calcein UltraBlue™ AM Working Solution
Prepare a Calcein UltraBlue™ AM working solution of 1 to 10 µM in the buffer of your choice (e.g., Hanks and Hepes buffer). For most cell lines, Calcein UltraBlue™ AM at the final concentration of 4 to 5 µM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.
Note     If your cells contain organic anion-transporters, probenecid (1–2.5 mM) or sulfinpyrazone (0.1–0.25 mM) may be added to the working solution to reduce leakage of the de-esterified indicators.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Prepare cells for imaging.
  2. Remove the cell culture medium and wash cells once with serum-free buffer to remove any remaining media.
    Note     Serum in cell culture media may contain esterase activity, which can increase background interference.
  3. Add Calcein UltraBlue™ AM working solution to the culture.
  4. Incubate cells at 37 °C for 30 to 60 minutes.
  5. Replace the dye working solution with HHBS or buffer of your choice (containing an anion transporter inhibitor, such as 1 mM probenecid, if applicable) to remove any excess probes.
  6. Measure the fluorescence intensity using either a fluorescence microscope equipped with a DAPI filter set, a flow cytometer equipped with a UV/violet laser and a 450/40 nm filter (Pacific Blue channel), or a fluorescence plate reader at Ex/Em = 360/450 nm cutoff 420 nm. 

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Calcein UltraBlue™ AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM124.583 µL622.913 µL1.246 mL6.229 mL12.458 mL
5 mM24.917 µL124.583 µL249.165 µL1.246 mL2.492 mL
10 mM12.458 µL62.291 µL124.583 µL622.913 µL1.246 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Excitation (nm)359
Emission (nm)458

Product Family


NameExcitation (nm)Emission (nm)
Calcein Red™ AM562576
Calcein Blue, AM *CAS 168482-84-6*354441
Calcein UltraGreen™ AM492514

Images


Citations


View all 19 citations: Citation Explorer
The zebrafish heart harbors a thermogenic beige fat depot analog of human epicardial adipose tissue
Authors: Morocho-Jaramillo, Paul-Andres and Kotlar-Goldaper, Ilan and Zakarauskas-Seth, Bhakti I and Purf{\"u}rst, Bettina and Filosa, Alessandro and Sawamiphak, Suphansa
Journal: Cell Reports (2024)
Double emulsion picoreactors for high-throughput single-cell encapsulation and phenotyping via FACS
Authors: Brower, Kara K and Khariton, Margarita and Suzuki, Peter H and Still, Chris and Kim, Gaeun and Calhoun, Suzanne GK and Qi, Lei S and Wang, Bo and Fordyce, Polly M
Journal: Analytical chemistry (2020): 13262--13270
Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang
Journal: Biomedical Optics Express (2017): 2599--2610
NINJ2--A novel regulator of endothelial inflammation and activation
Authors: Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined
Journal: Cellular Signalling (2017)
Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres
Journal: Scientific reports (2016)
Inhibition of ABC transport proteins by oil sands process affected water
Authors: Alharbi, Hattan A and Saunders, David MV and Al-Mousa, Ahmed and Alcorn, Jane and Pereira, Alberto S and Martin, Jonathan W and Giesy, John P and Wiseman, Steve B
Journal: Aquatic Toxicology (2016): 81--88
Rapid generation of collagen-based microtissues to study cell--matrix interactions
Authors: Brett, Marie-Elena and Crampton, Alex and ra L , undefined and Wood, David K
Journal: Technology (2016): 1--8
Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Alharbi, Hattan A and Alcorn, Jane and Al-Mousa, Ahmed and Giesy, John P and Wiseman, Steve B
Journal: Journal of Applied Toxicology (2016)
Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Thiebes, Anja Lena and Reddemann, Manuel Armin and Palmer, Johannes and Kneer, Reinhold and Jockenhoevel, Stefan and Cornelissen, Christian Gabriel
Journal: Tissue Engineering Part C: Methods (2016): 322--331
Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Liu, Peixi and Zhou, Yingjie and An, Qingzhu and Song, Yaying and Chen, Xi and Yang, Guo-Yuan and Zhu, Wei
Journal: MEDICINE (2016): 1--8

References


View all 84 references: Citation Explorer
Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine
Authors: Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.
Journal: Biophys J. (2006)
A vaccination and challenge model using calcein marked fish
Authors: Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.
Journal: Fish Shellfish Immunol (2006): 20
Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging
Authors: Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.
Journal: Cytometry A (2005): 78
Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake
Authors: Schoonen WG, Westerink WM, de Roos JA, Debiton E.
Journal: Toxicol In Vitro (2005): 505
Calcein AM release-based cytotoxic cell assay for fish leucocytes
Authors: Iwanowicz LR, Densmore CL, Ottinger CA.
Journal: Fish Shellfish Immunol (2004): 127
Calcein-AM is a detector of intracellular oxidative activity
Authors: Uggeri J, Gatti R, Belletti S, Sc and roglio R, Corradini R, Rotoli BM, Orl and ini G., undefined
Journal: Histochem Cell Biol (2004): 499
Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines
Authors: Mueller H, Kassack MU, Wiese M.
Journal: J Biomol Screen (2004): 506
In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue
Authors: Goto T, Kajiwara H, Yoshinari M, Fukuhara E, Kobayashi S, Tanaka T.
Journal: Biomaterials (2003): 3885
The effects of calcium chloride and sodium chloride on the electroporation-mediated skin permeation of fluorescein isothiocyanate (FITC)-dextrans in vitro
Authors: Tokudome Y, Sugibayashi K.
Journal: Biol Pharm Bull (2003): 1508