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Calcein UltraBlue™ AM
Calcein UltraBlue™ AM is a cell-permeable version of Calcein UltraBlue™. Upon getting into live cells the weakly fluorescent Calcein UltraBlue™ AM is hydrolyzed into Calcein UltraBlue™ that has the excitation/emission maxima similar to those of Calcein Blue, DAPI, Hoechst and AMCA. This exceptional spectral separation from the typical green and red fluorophores (such as FITC, TMR and Texas Red) provides additional options for multiplexing experiments. Calcein UltraBlue™ has similar spectral properties to those of Calcein Blue. However, compared with Calcein Blue, Calcein UltraBlue™ has higher photostability and stronger fluorescence intensity at physiological pH, making it a more robust fluorescent probe than Calcein Blue.
Fluorescence images of HeLa cells stained with Calcein UltraBlue™ AM (upper row) or Calcein Blue AM (lower row) in a Costar black wall/clear bottom 96-well plate. Left: Live HeLa cells in HH buffer; Middle: Live HeLa cells in medium; Right: Fixed HeLa cells.
Fluorescence images of HeLa cells stained with Calcein UltraBlue™ AM (upper row) or Calcein Blue AM (lower row) in a Costar black wall/clear bottom 96-well plate. Left: Live HeLa cells in HH buffer; Middle: Live HeLa cells in medium; Right: Fixed HeLa cells.
CatalogSize
Price
Quantity
2190810x50 ug
Price
 
Physical properties

Molecular weight802.68
SolventDMSO
Spectral properties

Excitation (nm)359
Emission (nm)458
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation350/405 nm laser
Emission450/40 nm filter
Instrument specification(s)Pacific Blue channel

Fluorescence microscope
ExcitationDAPI filter set
EmissionDAPI filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation360
Emission450
Cutoff420
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on January 23, 2025